质体转化中的未解决问题。
Unsolved problems in plastid transformation.
机构信息
Department of Soil, Plant, Environmental and Animal Production Sciences, University of Naples 'Federico II', Portici, Italy.
出版信息
Bioengineered. 2012 Nov-Dec;3(6):329-33. doi: 10.4161/bioe.21452. Epub 2012 Aug 15.
Abstract
Plants have been proved as a novel production platform for a wide range of biologically important compounds such as enzymes, therapeutic proteins, antibiotics, and proteins with immunological properties. In this context, plastid genetic engineering can be potentially used to produce recombinant proteins. However, several challenges still remain to be overcome if the full potential of plastid transformation technology is to be realized. They include the development of plastid transformation systems for species other than tobacco, the expression of transgenes in non-green plastids, the increase of protein accumulation and the appearance of pleiotropic effects. In this paper, we discuss the novel tools recently developed to overcome some limitations of chloroplast transformation.
摘要
植物已被证明是一个生产各种具有生物学重要性的化合物的新型平台，如酶、治疗蛋白、抗生素和具有免疫特性的蛋白。在这种情况下，质体遗传工程可被潜在地用于生产重组蛋白。然而，如果要充分发挥质体转化技术的潜力，仍有一些挑战需要克服。这些挑战包括为除烟草以外的物种开发质体转化系统，在非绿色质体中转基因的表达，增加蛋白积累和出现多效性效应。在本文中，我们讨论了最近开发的克服叶绿体转化一些局限性的新工具。
相似文献
1
Unsolved problems in plastid transformation.质体转化中的未解决问题。
Bioengineered. 2012 Nov-Dec;3(6):329-33. doi: 10.4161/bioe.21452. Epub 2012 Aug 15.
2
Production of foreign proteins using plastid transformation.利用质体转化生产外源蛋白。
Biotechnol Adv. 2012 Mar-Apr;30(2):387-97. doi: 10.1016/j.biotechadv.2011.07.019. Epub 2011 Aug 6.
3
Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials.马铃薯的质体转化：营养和工业材料的重要来源。
Methods Mol Biol. 2021;2317:247-256. doi: 10.1007/978-1-0716-1472-3_13.
4
Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro.块茎茄科植物质体的转化使β-葡糖苷酸酶在叶片和体外培养的微型块茎中的表达水平均显著提高。
Planta. 2012 Apr;235(4):807-18. doi: 10.1007/s00425-011-1541-6. Epub 2011 Nov 10.
5
Improvement of plastid transformation efficiency in potato by using vectors with homologous flanking sequences.通过使用具有同源侧翼序列的载体提高马铃薯质体转化效率
GM Crops. 2011 Apr-Jun;2(2):89-91. doi: 10.4161/gmcr.2.2.17504. Epub 2011 Apr 1.
6
High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.高效的叶绿体转化在马铃薯中实现，通过替代的 5' 和 3' 调控序列调节叶片和块茎中的转基因表达。
Transgenic Res. 2011 Feb;20(1):137-51. doi: 10.1007/s11248-010-9402-9. Epub 2010 May 13.
7
Stable transformation of the cotton plastid genome and maternal inheritance of transgenes.棉花质体基因组的稳定转化及转基因的母系遗传。
Plant Mol Biol. 2004 Sep;56(2):203-16. doi: 10.1007/s11103-004-2907-y.
8
Challenges and perspectives in commercializing plastid transformation technology.质体转化技术商业化中的挑战与前景
J Exp Bot. 2016 Nov;67(21):5945-5960. doi: 10.1093/jxb/erw360. Epub 2016 Oct 3.
9
Bacteriophage 5' untranslated regions for control of plastid transgene expression.噬菌体 5' 非翻译区对质体转基因表达的调控。
Planta. 2013 Feb;237(2):517-27. doi: 10.1007/s00425-012-1770-3. Epub 2012 Sep 30.
10
Determining the transgene containment level provided by chloroplast transformation.确定叶绿体转化提供的转基因遏制水平。
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):6998-7002. doi: 10.1073/pnas.0700008104. Epub 2007 Apr 9.
引用本文的文献
1
The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology.在高等植物中生产首个功能性抗体模拟物：叶绿体为癌症中的 HER2 成像生成 DARPin G3。
Biol Res. 2022 Oct 23;55(1):32. doi: 10.1186/s40659-022-00400-7.
2
Building customizable auto-luminescent luciferase-based reporters in plants.在植物中构建可定制的自动发光荧光素酶报告基因。
Elife. 2020 Mar 25;9:e52786. doi: 10.7554/eLife.52786.
3
Vitamin E Content and Composition in Tomato Fruits: Beneficial Roles and Bio-Fortification.番茄果实中的维生素E含量与组成：有益作用及生物强化
Int J Mol Sci. 2015 Dec 8;16(12):29250-64. doi: 10.3390/ijms161226163.
4
The HIV-1 Pr55 gag polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants.HIV-1 Pr55 gag多聚蛋白与质体膜结合，导致转基因烟草植株中叶绿体生物发生严重受损和幼苗致死。
Transgenic Res. 2015 Apr;24(2):319-31. doi: 10.1007/s11248-014-9845-5. Epub 2014 Oct 29.
5
A built-in strategy to mitigate transgene spreading from genetically modified corn.一种从转基因玉米中减轻转基因扩散的内置策略。
PLoS One. 2013 Dec 6;8(12):e81645. doi: 10.1371/journal.pone.0081645. eCollection 2013.
本文引用的文献
1
Identification of cis-elements conferring high levels of gene expression in non-green plastids.鉴定非绿色质体中高基因表达水平的顺式元件。
Plant J. 2012 Oct;72(1):115-28. doi: 10.1111/j.1365-313X.2012.05065.x. Epub 2012 Jul 17.
2
Exceptional inheritance of plastids via pollen in Nicotiana sylvestris with no detectable paternal mitochondrial DNA in the progeny.在野生烟草（Nicotiana sylvestris）中，通过花粉实现了质体的非凡遗传，后代中无法检测到父本线粒体 DNA。
Plant J. 2012 Oct;72(1):84-8. doi: 10.1111/j.1365-313X.2012.05057.x. Epub 2012 Jul 9.
3
Translational fusion and redirection to thylakoid lumen as strategies to improve the accumulation of a camelid antibody fragment in transplastomic tobacco.翻译融合及重定向至类囊体腔作为提高转基因烟草中骆驼科抗体片段积累量的策略。
Planta. 2012 Aug;236(2):703-14. doi: 10.1007/s00425-012-1642-x. Epub 2012 Apr 24.
4
Visual marker and Agrobacterium-delivered recombinase enable the manipulation of the plastid genome in greenhouse-grown tobacco plants.可见标记和农杆菌介导的重组酶可用于在温室中生长的烟草植物中操纵质体基因组。
Plant J. 2012 May;70(4):717-25. doi: 10.1111/j.1365-313X.2012.04918.x. Epub 2012 Mar 6.
5
Increased accumulation and stability of rotavirus VP6 protein in tobacco chloroplasts following changes to the 5' untranslated region and the 5' end of the coding region.改变 5'非翻译区和编码区 5'端后，烟草叶绿体中轮状病毒 VP6 蛋白的积累和稳定性增加。
Plant Biotechnol J. 2012 May;10(4):422-34. doi: 10.1111/j.1467-7652.2011.00675.x. Epub 2012 Jan 19.
6
Metabolic adaptation in transplastomic plants massively accumulating recombinant proteins.转叶绿体植物中重组蛋白大量积累的代谢适应。
PLoS One. 2011;6(9):e25289. doi: 10.1371/journal.pone.0025289. Epub 2011 Sep 22.
7
Improvement of plastid transformation efficiency in potato by using vectors with homologous flanking sequences.通过使用具有同源侧翼序列的载体提高马铃薯质体转化效率
GM Crops. 2011 Apr-Jun;2(2):89-91. doi: 10.4161/gmcr.2.2.17504. Epub 2011 Apr 1.
8
Production of foreign proteins using plastid transformation.利用质体转化生产外源蛋白。
Biotechnol Adv. 2012 Mar-Apr;30(2):387-97. doi: 10.1016/j.biotechadv.2011.07.019. Epub 2011 Aug 6.
9
Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop.苜蓿叶绿体的转化及绿色荧光蛋白在饲料作物中的表达。
Biotechnol Lett. 2011 Dec;33(12):2487-94. doi: 10.1007/s10529-011-0709-2. Epub 2011 Jul 23.
10
Biolistic co-transformation of the nuclear and plastid genomes.生物弹道共转化核和质体基因组。
Plant J. 2011 Sep;67(5):941-8. doi: 10.1111/j.1365-313X.2011.04631.x. Epub 2011 Jun 24.
Zotero 插件