CNR-IGV, Institute of Plant Genetics, Portici (NA), Italy.
Biotechnol Adv. 2012 Mar-Apr;30(2):387-97. doi: 10.1016/j.biotechadv.2011.07.019. Epub 2011 Aug 6.
In the past decades, the progress made in plant biotechnology has made possible the use of plants as a novel production platform for a wide range of molecules. In this context, the transformation of the plastid genome has given a huge boost to prove that plants are a promising system to produce recombinant proteins. In this review, we provide a background on plastid genetics and on the principles of this technology in higher plants. Further, we discuss the most recent biotechnological applications of plastid transformation for the production of enzymes, therapeutic proteins, antibiotics, and proteins with immunological properties. We also discuss the potential of plastid biotechnology and the novel tools developed to overcome some limitations of chloroplast transformation.
在过去几十年中，植物生物技术的进步使得利用植物作为生产各种分子的新型平台成为可能。在这种情况下，质体基因组的转化极大地证明了植物是生产重组蛋白的有前途的系统。在这篇综述中，我们提供了质体遗传学的背景以及高等植物中该技术的原理。此外，我们讨论了质体转化在生产酶、治疗蛋白、抗生素和具有免疫特性的蛋白方面的最新生物技术应用。我们还讨论了质体生物技术的潜力和为克服叶绿体转化的一些限制而开发的新工具。
