Postgraduate Program for Specialized Endodontics, Faculty of Medical Sciences, School of Dentistry, University of El Salvador, Buenos Aires, Argentina.
J Endod. 2012 Sep;38(9):1233-8. doi: 10.1016/j.joen.2012.05.010. Epub 2012 Jul 6.
The purpose of this study was to evaluate the subcutaneous connective tissue reaction in rats to a mineral trioxide aggregate (MTA)-based endodontic sealer Fillapex (Angelus, Londrina, PR, Brazil) and compare it with Grossman sealer (Farmadental, Buenos Aires, Argentina).
Sterile medical-grade silicone tubes containing the test materials were implanted in 24 Wistar rats. After 10, 30, and 90 days, the animals (n = 8 per period) were euthanized, and the implants along with their surrounding tissues were dissected, fixed, and processed for histologic evaluation. A 4-category evaluation system was used to evaluate the microscopic observations. The tissue response on the lateral walls of the silicone tubes was used as the negative control. The data were analyzed for statistical significance using the Wilcoxon signed rank, Kruskal-Wallis, and Dunn tests.
Fillapex showed a severe tissue reaction for all 3 observation periods. Grossman sealer showed similar features after 10 and 30 days, but the reaction decreased slightly after 90 days. In contrast, the negative controls did not show adverse reactions in any sample of the 3 time periods. After 10 and 30 days, no statistically significant differences were found between Fillapex and Grossman sealer (P > .05); however, the difference was significant after 90 days (P < .05). For all experimental periods, there were statistically significant differences between both Fillapex and Grossman sealer and the negative controls (P < .05).
It was concluded that both MTA-Fillapex and Grossman sealer remained toxic to subcutaneous tissues in rats after 90 days.
本研究旨在评估大鼠皮下结缔组织对矿物三氧化物聚合体（MTA）基根管封闭剂 Fillapex（Angelus，Londrina，PR，巴西）的反应，并将其与 Grossman 封闭剂（Farmadental，Buenos Aires，Argentina）进行比较。
将含有测试材料的无菌医用级硅酮管植入 24 只 Wistar 大鼠体内。在第 10、30 和 90 天，处死动物（每个时间点 8 只），并解剖植入物及其周围组织，进行固定和组织学评估。使用 4 级评估系统评估微观观察结果。硅酮管侧壁的组织反应作为阴性对照。使用 Wilcoxon 符号秩检验、Kruskal-Wallis 检验和 Dunn 检验对数据进行统计显著性分析。
Fillapex 在所有 3 个观察期均表现出严重的组织反应。Grossman 封闭剂在第 10 和 30 天表现出相似的特征，但在第 90 天反应略有减轻。相比之下，在 3 个时间点的任何样本中，阴性对照均未出现不良反应。在第 10 和 30 天，Fillapex 和 Grossman 封闭剂之间无统计学差异（P>.05）；然而，在第 90 天差异显著（P<.05）。对于所有实验期，Fillapex 和 Grossman 封闭剂与阴性对照之间均有统计学差异（P<.05）。
在第 90 天，MTA-Fillapex 和 Grossman 封闭剂对大鼠皮下组织仍具有毒性。
