Dahake Prasanna T, Joshi Sphurti S, Kale Yogesh J, Dadpe Mahesh V, Kendre Shrikant B
Department of Paediatric and Preventive Dentistry, Maharashtra Institute of Dental Sciences & Research (Dental College & Hospital), Latur, Maharashtra, India.
Int J Clin Pediatr Dent. 2023 May-Jun;16(3):431-436. doi: 10.5005/jp-journals-10005-2531.
The present study was intended to assess the biocompatibility of newly formulated materials such as zinc oxide (ZO) admixed with ajwain oil (A) as well as ajwain eugenol (E) (1:1 ratio) against ZOE through an animal model as root canal obturating materials in deciduous teeth.
The study involved randomly selected 24 albino rats, which were divided into three groups based on test materials. Two polyethylene tubes (PETT) (8 mm long × 1 mm internal diameter) were inserted into connective tissues of the dorsal side on either side of each rat empty tube (negative control) and another containing test material (test group). Animals were sacrificed at the end of the 7th and 21st days. PETT with surrounding connective tissues were excised. Histopathological evaluations of the material's biocompatibility were done by determining inflammatory tissue responses. Non-parametric tests such as Kruskal-Wallis and Mann-Whitney were used for statistical analysis ( < 0.05).
Histopathological examination on the 7th day showed increased polymorphonuclear cells for all test materials compared to the negative control ( = 0.92), suggesting acute inflammation. The inflammation subsided gradually after 21 days ( = 0.48). The lymphocytes increased after 21 days for all the materials indicating chronic inflammation ( = 0.79), as well as fibroblasts ( = 0.34) and capillaries ( = 0.35), indicating healing and repair.
The newly formulated obturating materials were found to be biocompatible compared to ZOE.
Dahake PT, Joshi SS, Kale YJ, Biocompatibility of Oil Combined with Eugenol and Zinc Oxide as a Deciduous Root Canal Obturating Material: An Study. Int J Clin Pediatr Dent 2023;16(3):431-436.
本研究旨在通过动物模型评估新配制的材料（如氧化锌（ZO）与阿育吠陀油（A）以及阿育吠陀丁香油（E）按1:1比例混合）作为乳牙根管充填材料相对于氧化锌丁香油糊剂（ZOE）的生物相容性。
该研究随机选取24只白化大鼠，根据测试材料分为三组。将两根聚乙烯管（PETT）（长8毫米×内径1毫米）插入每只大鼠背部两侧的结缔组织中，一根为空管（阴性对照），另一根装有测试材料（测试组）。在第7天和第21天结束时处死动物。切除带有周围结缔组织的PETT。通过确定炎症组织反应对材料的生物相容性进行组织病理学评估。使用非参数检验如Kruskal-Wallis和Mann-Whitney进行统计分析（P<0.05）。
第7天的组织病理学检查显示，与阴性对照相比，所有测试材料的多形核细胞均增加（P = 0.92），提示急性炎症。21天后炎症逐渐消退（P = 0.48）。21天后所有材料的淋巴细胞均增加，提示慢性炎症（P = 0.79），同时成纤维细胞（P = 0.34）和毛细血管（P = 0.35）增加，提示愈合和修复。
与氧化锌丁香油糊剂相比，新配制的充填材料具有生物相容性。
Dahake PT, Joshi SS, Kale YJ, 油与丁香酚和氧化锌联合作为乳牙根管充填材料的生物相容性：一项动物研究。《国际临床儿科牙科学杂志》2023；16(3):431 - 436。
