Nanobioelectronics & Biosensors Group, ICN-CSIC/Catalan Institute of Nanotechnology, Campus de la UAB, Bellaterra, Barcelona, Spain.
Small. 2012 Dec 7;8(23):3605-12. doi: 10.1002/smll.201201205. Epub 2012 Aug 15.
A rapid cancer cell detection and quantification assay, based on the electrocatalytic properties of gold nanoparticles towards the hydrogen evolution reaction, is described. The selective labeling of cancer cells is performed in suspension, allowing a fast interaction between the gold nanoparticle labels and the target proteins expressed at the cell membrane. The subsequent electrochemical detection is accomplished with small volumes of sample and user-friendly equipment through a simple electrochemical method that generates a fast electrochemical response used for the quantification of nanoparticle-labeled cancer cells. The system establishes a selective cell-detection assay capable of detecting 4 × 10(3) cancer cells in suspension that can be extended to several other cells detection scenarios.
本文描述了一种基于金纳米粒子对析氢反应的电催化性质的快速癌细胞检测和定量分析方法。在悬浮液中进行癌细胞的选择性标记，允许金纳米粒子标记物与细胞膜上表达的靶蛋白快速相互作用。随后通过一种简单的电化学方法进行电化学检测，该方法生成快速的电化学响应，用于定量检测纳米粒子标记的癌细胞。该系统建立了一种选择性的细胞检测分析方法，能够检测悬浮液中的 4×10(3)个癌细胞，并且可以扩展到其他几种细胞检测场景。
