Nanobioelectronics & Biosensors Group, Catalan Institute of Nanotechnology, CIN2 (ICN-CSIC), Campus de la UAB, [corrected] Barcelona, Spain.
Anal Chem. 2009 Dec 15;81(24):10268-74. doi: 10.1021/ac902087k.
There is a high demand for simple, rapid, efficient, and user-friendly alternative methods for the detection of cells in general and, in particular, for the detection of cancer cells. A biosensor able to detect cells would be an all-in-one dream device for such applications. The successful integration of nanoparticles into cell detection assays could allow for the development of this novel class of cell sensors. Indeed, their application could well have a great future in diagnostics, as well as other fields. As an example of a novel biosensor, we report here an electrocatalytic device for the specific identification of tumor cells that quantifies gold nanoparticles (AuNPs) coupled with an electrotransducing platform/sensor. Proliferation and adherence of tumor cells are achieved on the electrotransducer/detector, which consists of a mass-produced screen-printed carbon electrode (SPCE). In situ identification/quantification of tumor cells is achieved with a detection limit of 4000 cells per 700 microL of suspension. This novel and selective cell-sensing device is based on the reaction of cell surface proteins with specific antibodies conjugated with AuNPs. Final detection requires only a couple of minutes, taking advantage of the catalytic properties of AuNPs on hydrogen evolution. The proposed detection method does not require the chemical agents used in most existing assays for the detection of AuNPs. It allows for the miniaturization of the system and is much cheaper than other expensive and sophisticated methods used for tumor cell detection. We envisage that this device could operate in a simple way as an immunosensor or DNA sensor. Moreover, it could be used, even by inexperienced staff, for the detection of protein molecules or DNA strands.
人们迫切需要简单、快速、高效且易于使用的替代方法来检测细胞，特别是癌细胞。能够检测细胞的生物传感器将成为此类应用的理想全能设备。成功地将纳米粒子整合到细胞检测分析中，可以开发出这种新型细胞传感器。实际上，它们的应用在诊断领域以及其他领域都具有广阔的前景。作为新型生物传感器的一个示例，我们在这里报告了一种用于特异性识别肿瘤细胞的电化学生物传感器，该传感器可以定量结合金纳米粒子（AuNPs）的电转导平台/传感器。肿瘤细胞的增殖和黏附在电转导/检测器上实现，该电转导/检测器由大量生产的丝网印刷碳电极（SPCE）组成。通过与 AuNPs 结合的特异性抗体与细胞表面蛋白的反应，可以实现对肿瘤细胞的原位识别/定量，检测限为每 700 μL 悬浮液 4000 个细胞。这种新型的、选择性的细胞传感装置基于细胞表面蛋白与特异性抗体结合的 AuNPs 的反应。最终检测仅需几分钟的时间，利用 AuNPs 在析氢反应中的催化特性。与大多数用于检测 AuNPs 的现有检测方法相比，所提出的检测方法不需要使用化学试剂。它允许系统小型化，并且比用于肿瘤细胞检测的其他昂贵且复杂的方法便宜得多。我们设想该装置可以简单地作为免疫传感器或 DNA 传感器运行。此外，即使是没有经验的人员也可以使用它来检测蛋白质分子或 DNA 链。
