Center for Medical Physics and Biomedical Engineering, Vienna, Austria.
Invest Ophthalmol Vis Sci. 2012 Sep 12;53(10):6062-71. doi: 10.1167/iovs.12-9514.
The present experiments were undertaken to evaluate the validity of absolute flow velocity measurements with a dual-beam bidirectional Doppler Fourier-domain optical coherence tomography (FD-OCT) system.
The flow velocities of diluted milk through a glass capillary were measured at 30 different preset velocities in the range of 0.9 to 39.3 mm/s by bidirectional Doppler FD-OCT. The flow through the capillary was controlled by two infusion pumps working in different flow ranges and based on different technical principles. In vivo the validity of the method for measuring blood flow in retinal vessels was tested at bifurcations. The continuity equation was verified at 10 retinal venous bifurcations of 10 young healthy subjects (mean age, 29 ± 3 years) by velocity measurements, using dual-beam bidirectional Doppler FD-OCT, and measurements of retinal diameters, using the Dynamic Vessel Analyzer.
Flow velocities as measured with bidirectional Doppler FD-OCT in the glass capillary were in good agreement with the preset velocities (r = 0.994, P < 0.001 each). No significant difference was found between flow in the trunk vessels before the bifurcation (11.3 ± 5.2 μL/min) and the sum of flows in the daughter vessels (10.7 ± 4.8 μL/min). A significant association was found between retinal vessel diameters and both retinal blood velocities (r = 0.72, P < 0.001) and retinal blood flow (r = 0.95, P < 0.0001).
Dual-beam bidirectional Doppler FD-OCT delivered accurate retinal blood velocity values and, thus, offers high potential for examination of retinal blood flow in ocular disease.
本实验旨在评估双光束双向多普勒傅里叶域光学相干断层扫描（FD-OCT）系统测量绝对流速的有效性。
通过双光束双向多普勒 FD-OCT，在 0.9 至 39.3mm/s 的 30 个预设流速范围内，测量通过玻璃毛细管的稀释牛奶的流速。通过两个以不同流速范围和不同技术原理工作的输液泵控制流过毛细管的流速。在体内，通过在 10 名年轻健康受试者（平均年龄 29±3 岁）的视网膜血管的分支处测量血流，验证该方法测量视网膜血管血流的有效性。使用双光束双向多普勒 FD-OCT 测量流速，并使用动态血管分析仪测量视网膜直径，以验证连续方程。
玻璃毛细管中双光束双向多普勒 FD-OCT 测量的流速与预设流速非常吻合（r = 0.994，P <0.001）。在分支前主干血管中的血流（11.3±5.2μL/min）与分支后子血管中的血流之和（10.7±4.8μL/min）之间无显著差异。视网膜血管直径与视网膜血流速度（r = 0.72，P <0.001）和视网膜血流量（r = 0.95，P <0.0001）均呈显著相关。
双光束双向多普勒 FD-OCT 提供了准确的视网膜血流速度值，因此在眼部疾病的视网膜血流检查中具有很大的潜力。
