Told Reinhard, Wang Lin, Cull Grant, Thompson Simon J, Burgoyne Claude F, Aschinger Gerold C, Schmetterer Leopold, Werkmeister René M
Center for Medical Physics and Biomedical Engineering Medical University of Vienna, Vienna, Austria 2Devers Eye Institute, Legacy Research Institute, Portland, Oregon, United States.
Devers Eye Institute, Legacy Research Institute, Portland, Oregon, United States.
Invest Ophthalmol Vis Sci. 2016 Mar;57(3):1432-40. doi: 10.1167/iovs.16-19140.
We validated noninvasive Doppler-optical coherence tomography (OCT) blood flow measurements against the terminal microsphere method in a surgical induced optic nerve transection nonhuman primate model.
In 6 nonhuman primates, total retinal blood flow (TRBF) was measured with a custom-built dual-beam bidirectional Doppler Fourier Domain (FD)-OCT. Peripapillary retinal nerve fiber layer thickness (RNFLT) was measured by Spectralis spectral-domain (SD)-OCT. Measurements were performed every 10 to 15 days before and after unilateral optic nerve transection (ONT) until RNFLT was reduced by more than 40% from baseline. Before the animals were killed, TRBF was measured using the microsphere technique.
A significant correlation between all arterial and venous Doppler OCT TRBF measurements was found in ONT and contralateral control eyes (both P < 0.01, n = 6). The Bland-Altman analysis showed a bias of 0.57 in the ONT group and 0.02 in the contralateral control group. Also, excellent agreement was observed between Doppler OCT and microsphere measurements (P < 0.01, r = 0.976, bias = 0.54). After ONT, TRBF and RNFLT decreased by -51% ± 42% and -44% ± 2% (n = 5), respectively. In the contralateral control eyes, TRBF and RNFLT were unchanged.
Very good accordance was found between TRBF measurements, obtained with dual-beam bidirectional Doppler FD-OCT and the microsphere method. It also was possible to monitor changes over time in TRBF after ONT with Doppler OCT. These findings highlight the accuracy and potential of noninvasive Doppler OCT to provide valuable information for detecting early changes in ocular disease in future.
在手术诱导的视神经横断非人灵长类动物模型中，我们对照终末微球法验证了无创多普勒光学相干断层扫描（OCT）血流测量。
在6只非人灵长类动物中，使用定制的双光束双向多普勒傅里叶域（FD）-OCT测量视网膜总血流（TRBF）。通过Spectralis光谱域（SD）-OCT测量视乳头周围视网膜神经纤维层厚度（RNFLT）。在单侧视神经横断（ONT）前后每10至15天进行测量，直至RNFLT比基线降低超过40%。在处死动物前，使用微球技术测量TRBF。
在ONT眼和对侧对照眼中，所有动脉和静脉多普勒OCT TRBF测量值之间均存在显著相关性（均P<0.01，n = 6）。Bland-Altman分析显示ONT组偏差为0.57，对侧对照组偏差为0.02。此外，在多普勒OCT和微球测量之间观察到极好的一致性（P<0.01，r = 0.976，偏差 = 0.54）。ONT后，TRBF和RNFLT分别下降了-51%±42%和-44%±2%（n = 5）。在对侧对照眼中，TRBF和RNFLT未发生变化。
双光束双向多普勒FD-OCT获得的TRBF测量值与微球法之间具有很好的一致性。使用多普勒OCT还可以监测ONT后TRBF随时间的变化。这些发现突出了无创多普勒OCT在未来为检测眼部疾病早期变化提供有价值信息的准确性和潜力。
