Matthieu J M, Amiguet P
Laboratoire de Neurochimie, Centre Hospitalier Universitaire Vaudois, Lausanne, Suisse.
Dev Neurosci. 1990;12(4-5):293-302. doi: 10.1159/000111858.
The myelin/oligodendrocyte glycoprotein (MOG) is identified by monoclonal antibody 8-18C5. MOG is localized on the surface of myelin and oligodendrocyte processes. Recently, several studies have shown that MOG plays an important role as a target for antibody-induced demyelination. In the present study, we investigated MOG expression in the brains of normal and myelin-deficient (mld) mutant mice during development. By gel electrophoresis and immunoblotting, we observed the developmental pattern of two closely migrating bands, with apparent molecular masses of 26 and 28 kilodaltons. Their concentrations increased coordinately during the most active phase of myelin and myelin basic protein (MBP) synthesis. Between 20 and 25 days of age, the MOG developmental pattern superimposed that of MBP as well as myelin yields. In mld mutant mice, which are affected by a severe deficit of MBP synthesis, MOG was present at reduced levels (40% of controls at 60 days of age). At 85 days of age, mld mice exhibited increased concentrations of MBP, and myelin was better compacted. At this age, MOG concentrations increased and reached 70% of controls. These results suggest that MOG could play a role in the maintenance or completion of the myelin sheath. Its expression level may be modulated by the presence of compact myelin and/or MBP in the myelin sheath.
髓鞘/少突胶质细胞糖蛋白（MOG）由单克隆抗体8-18C5识别。MOG定位于髓鞘和少突胶质细胞突起的表面。最近，多项研究表明，MOG作为抗体诱导的脱髓鞘作用的靶点发挥着重要作用。在本研究中，我们调查了正常和髓鞘缺陷（mld）突变小鼠在发育过程中大脑中MOG的表达情况。通过凝胶电泳和免疫印迹，我们观察到两条迁移紧密的条带的发育模式，其表观分子量分别为26和28千道尔顿。在髓鞘和髓鞘碱性蛋白（MBP）合成最活跃的阶段，它们的浓度协同增加。在20至25日龄之间，MOG的发育模式与MBP以及髓鞘产量的模式重叠。在受MBP合成严重缺陷影响的mld突变小鼠中，MOG的水平降低（60日龄时为对照组的40%）。在85日龄时，mld小鼠的MBP浓度增加，髓鞘更紧密地压实。在这个年龄，MOG浓度增加并达到对照组的70%。这些结果表明，MOG可能在髓鞘的维持或完成中发挥作用。其表达水平可能受髓鞘中紧密髓鞘和/或MBP的存在调节。
