Solly S K, Thomas J L, Monge M, Demerens C, Lubetzki C, Gardinier M V, Matthieu J M, Zalc B
Laboratoire de Neurobiologie Cellulaire, Moléculaire, et Clinique INSERM U-134, Hôpital de la Salpêtrière, Université Pierre et Marie Curie, Paris, France.
Glia. 1996 Sep;18(1):39-48. doi: 10.1002/(SICI)1098-1136(199609)18:1<39::AID-GLIA4>3.0.CO;2-Z.
We investigated the onset of expression of the myelin/oligodendrocyte glycoprotein (MOG) mRNA and protein in the developing mouse central nervous system. In situ hybridization on brain sections at different stages of embryonic and postnatal development showed that MOG transcripts were first detected at birth in the medulla oblongata. During the first week after birth, cells expressing MOG mRNA were located in the ventral longitudinal funiculus. During the second postnatal week, the pattern of MOG mRNA expression extended rostrally to the mid-forebrain regions and reached completion by the beginning of the third week. MOG transcription was delayed by several days with respect to myelin basic protein (MBP), and it appeared that while the MBP probe labeled both non-myelinating and myelinating oligodendrocytes, only the latter were MOG-positive. In vitro, immunocytochemical analysis of MOG protein expression, performed on myelinating cultures derived from mouse brain embryos at 15 days of gestation, confirmed the strict restriction of MOG expression to myelinating oligodendrocytes. In particular, oligodendrocytes lining up their processes along axons, but not yet having started to deposit a myelin sheath, were still MOG negative. However, in the same cultures, pseudo-myelinating oligodendrocytes (i.e., cells not associated with neurites, but forming whorls of myelin-like figures) were MOG positive. Similarly, rat CG4 cells, an oligodendrocyte-like cell line, expressed MOG only after they had extended sheet-like processes, which suggested that the activation of MOG transcription depends more on an intrinsic oligodendroglial maturation program of myelination than on a neuronal signal.
我们研究了髓鞘/少突胶质细胞糖蛋白（MOG）mRNA和蛋白在发育中的小鼠中枢神经系统中的表达起始情况。对胚胎期和出生后不同发育阶段的脑切片进行原位杂交显示，MOG转录本最早在出生时于延髓中被检测到。在出生后的第一周，表达MOG mRNA的细胞位于腹侧纵索。在出生后的第二周，MOG mRNA的表达模式向前延伸至中脑前区，并在第三周开始时完成。与髓鞘碱性蛋白（MBP）相比，MOG转录延迟了几天，并且似乎虽然MBP探针标记了非髓鞘形成和髓鞘形成的少突胶质细胞，但只有后者是MOG阳性的。在体外，对妊娠15天的小鼠脑胚胎来源的髓鞘形成培养物进行MOG蛋白表达的免疫细胞化学分析，证实了MOG表达严格局限于髓鞘形成的少突胶质细胞。特别是，沿着轴突排列其突起但尚未开始沉积髓鞘的少突胶质细胞仍然是MOG阴性的。然而，在相同的培养物中，假髓鞘形成的少突胶质细胞（即不与神经突相关但形成髓鞘样结构的细胞）是MOG阳性的。同样，大鼠CG4细胞系，一种少突胶质细胞样细胞系，只有在它们伸出片状突起后才表达MOG，这表明MOG转录的激活更多地取决于少突胶质细胞内在的髓鞘形成成熟程序，而不是神经元信号。
