Nankai University, The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics and TEDA Applied Physics School, Tianjin 300071, China.
J Biomed Opt. 2012 Jul;17(7):075011. doi: 10.1117/1.JBO.17.7.075011.
We investigate the effect of tissue fluid on the measurement of complex refractive index (RI) of animal tissue. A new model is proposed and verified through experimental results of simulation samples made of glycerol and methyl-red-doped poly(methyl methacrylate). Coupled with polarized optical reflectance measurements performed on several kinds of animal muscle tissues, RIs were resolved using the new model. We find that the tissue fluid existing at the prism-sample interface is unavoidable. We also find that with a change of proportion of the tissue fluid, the RI of muscle tissue can still be measured using the new model.
我们研究了组织液对动物组织复折射率测量的影响。通过甘油和甲基红掺杂聚甲基丙烯酸甲酯模拟样品的实验结果，提出并验证了一种新模型。结合几种动物肌肉组织的偏振光反射测量，利用新模型解析了复折射率。我们发现棱镜-样品界面处存在组织液是不可避免的。我们还发现，随着组织液比例的变化，利用新模型仍然可以测量肌肉组织的复折射率。
