Zeng Huanhuan, Wang Jin, Ye Qing, Deng Zhichao, Mei Jianchun, Zhou Wenyuan, Zhang Chunping, Tian Jianguo
Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of physics and TEDA Applied Physics School, Nankai University, Tianjin 300071, China.
Advanced Technology Institute, Nankai University, Tianjin 300071, China.
Biomed Opt Express. 2014 Sep 8;5(10):3482-93. doi: 10.1364/BOE.5.003482. eCollection 2014 Oct 1.
In recent years, the tissue optical clearing (OC) technique in the biomedicine field has drawn lots of attention. Various physical and chemical methods have been introduced to improve the efficacy of OC. In this study, the effect of the combination of glycerol and ultrasound treatment on OC of in vitro porcine muscle tissues has been investigated. The refractive index (RI) matching mechanism of OC was directly observed based on the derivative total reflection method. A theoretical model was used to simulate the proportion of tissue fluid in the illuminated area. Moreover, the total transmittance spectra have been obtained by a spectrometer over the range from 450 nm to 700 nm. The administration of glycerol and ultrasound has led to an increase of the RI of background medium and a more RI matching environment was achieved. The experimental results support the validity of the ultrasound treatment for OC. The RI matching mechanism has been firstly quantitatively analyzed based on the derivative total reflection method.
近年来，生物医学领域的组织光学透明化（OC）技术备受关注。人们引入了各种物理和化学方法来提高OC的效果。在本研究中，研究了甘油和超声处理相结合对体外猪肌肉组织OC的影响。基于导数全反射法直接观察了OC的折射率（RI）匹配机制。使用理论模型模拟照明区域中组织液的比例。此外，通过光谱仪在450nm至700nm范围内获得了总透射光谱。甘油和超声的施用导致背景介质的RI增加，并实现了更匹配RI的环境。实验结果支持超声处理对OC的有效性。首次基于导数全反射法对RI匹配机制进行了定量分析。
