RIKEN, Nanophotonics Laboratory, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
J Biomed Opt. 2012 Jul;17(7):076001. doi: 10.1117/1.JBO.17.7.076001.
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.
我们首次展示了深紫外（DUV）拉曼成像在细胞中的应用。在 257nm 激发下，通过共振带可以观察到 HeLa 细胞中核苷酸的分布，这些共振带归因于鸟嘌呤和腺嘌呤。获得的图像代表了细胞核中核仁处的 DNA 定位和细胞质中 RNA 的分布。该技术扩展了拉曼显微镜作为一种工具的潜力，由于共振，它可以选择性地探测细胞中的核酸，具有高灵敏度。
