Department of Chemistry and School of Analytical Sciences Adlershof, Humboldt-Universität zu Berlin, Berlin, Germany.
ISAS Berlin, Berlin, Germany.
Appl Spectrosc. 2021 Aug;75(8):994-1002. doi: 10.1177/00037028211025575. Epub 2021 Jul 2.
The ultraviolet resonance Raman spectra of the adenine-containing enzymatic redox cofactors nicotinamide adenine dinucleotide and flavin adenine dinucleotide in aqueous solution of physiological concentration are compared with the aim of distinguishing between them and their building block adenine in potential co-occurrence in biological materials. At an excitation wavelength of 266 nm, the spectra are dominated by the strong resonant contribution from adenine; nevertheless, bands assigned to vibrational modes of the nicotinamide and the flavin unit are found to appear at similar signal strength. Comparison of spectra measured at pH 7 with data obtained pH 10 and pH 3 shows characteristic changes when pH is increased or lowered, mainly due to deprotonation of the flavin and nicotinamide moieties, and protonation of the adenine, respectively.
比较了含腺嘌呤的酶促氧化还原辅因子烟酰胺腺嘌呤二核苷酸和黄素腺嘌呤二核苷酸在生理浓度水溶液中的紫外共振拉曼光谱，目的是在潜在共存的生物材料中区分它们及其结构单元腺嘌呤。在 266nm 的激发波长下，光谱主要由腺嘌呤的强共振贡献主导；然而，发现分配给烟酰胺和黄素单元的振动模式的谱带以相似的信号强度出现。比较在 pH 7 下测量的光谱与在 pH 10 和 pH 3 下获得的数据表明，当 pH 升高或降低时会出现特征变化，主要归因于黄素和烟酰胺部分的去质子化以及腺嘌呤的质子化。
