University Health Network, Ontario Cancer Institute, Toronto, Ontario, Canada.
J Biomed Opt. 2012 Jul;17(7):076017. doi: 10.1117/1.JBO.17.7.076017.
As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that do not overlap significantly with endogenous Raman signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM) concentrations of NPs against a broad tissue autofluorescence background in wide-field mode, with short integration times that compare favorably with point-by-point mapping typically used in SERS imaging. This advance will facilitate the potential applications of SERS NPs as contrast agents in wide-field multiplexed biomarker-targeted imaging in vivo.
随着分子成像技术朝着更低的检测极限发展，消除内源性背景信号变得至关重要。我们提出了一种简单的背景抑制技术，可以特异性地将表面增强拉曼散射（SERS）活性纳米粒子（NPs）的信号与体内组织自发荧光背景区分开来。SERS NPs 具有极其狭窄的光谱峰，与内源性拉曼信号没有明显重叠。这可以利用特定的窄带滤波器来进行成像，以在广角模式下以 picomolar（pM）浓度的 NPs 对广泛的组织自发荧光背景进行成像，同时具有较短的积分时间，这与 SERS 成像中通常使用的逐点映射相比具有优势。这一进展将促进 SERS NPs 作为对比剂在体内宽场复用生物标志物靶向成像中的潜在应用。
