Department of Radiology, Stanford University, Stanford, CA, USA.
Department of Bioengineering, Stanford University, Stanford, CA, USA.
Methods Mol Biol. 2021;2350:331-340. doi: 10.1007/978-1-0716-1593-5_21.
Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are ideal multiplexing probes for in vivo imaging and tissue staining. Their remarkable sensitivity and unique Raman molecular fingerprint results in minimal background compared to other optical modalities. These characteristics also allow multiplexing down to the attomolar concentration. Here we describe the synthesis and in vivo multiplexing application of a SERS NP library.
表面增强拉曼散射（SERS）纳米粒子（NPs）是用于体内成像和组织染色的理想多重标记探针。与其他光学模式相比，它们具有显著的灵敏度和独特的拉曼分子指纹，因此背景信号非常低。这些特性还允许达到飞摩尔浓度的多重标记。本文描述了 SERS NP 文库的合成及其在体内的多重标记应用。
