Wang Yu W, Khan Altaz, Leigh Steven Y, Wang Danni, Chen Ye, Meza Daphne, Liu Jonathan T C
Department of Biomedical Engineering, Stony Brook University (SUNY), Stony Brook, NY 11794 USA ; Current institution: Department of Mechanical Engineering, University of Washington, Seattle, WA 98195 USA ; These authors contributed equally to this work.
Department of Biomedical Engineering, Stony Brook University (SUNY), Stony Brook, NY 11794 USA ; These authors contributed equally to this work.
Biomed Opt Express. 2014 Aug 1;5(9):2883-95. doi: 10.1364/BOE.5.002883. eCollection 2014 Sep 1.
The early detection and biological investigation of esophageal cancer would benefit from the development of advanced imaging techniques to screen for the molecular changes that precede and accompany the onset of cancer. Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have the potential to improve cancer detection and the investigation of cancer progression through the sensitive and multiplexed phenotyping of cell-surface biomarkers. Here, a miniature endoscope featuring rotational scanning and axial pull back has been developed for 2D spectral imaging of SERS NPs topically applied on the lumenal surface of the rat esophagus. Raman signals from low-pM concentrations of SERS NP mixtures are demultiplexed in real time to accurately calculate the concentration and ratio of the NPs. Ex vivo and in vivo experiments demonstrate the feasibility of topical application and imaging of multiplexed SERS NPs along the entire length of the rat esophagus.
食管癌的早期检测和生物学研究将受益于先进成像技术的发展，以筛查癌症发生之前和伴随发生的分子变化。表面增强拉曼散射（SERS）纳米颗粒（NPs）有潜力通过对细胞表面生物标志物进行灵敏且多重的表型分析来改善癌症检测和癌症进展研究。在此，已开发出一种具有旋转扫描和轴向回拉功能的微型内窥镜，用于对局部应用于大鼠食管腔内表面的SERS NPs进行二维光谱成像。来自低皮摩尔浓度SERS NP混合物的拉曼信号被实时解复用，以准确计算NPs的浓度和比例。体外和体内实验证明了在大鼠食管全长上局部应用和成像多重SERS NPs的可行性。
