人脑血管紧张素转换酶与脑啡肽降解活性的分离。

Separation of human brain angiotensin-converting enzyme from enkephalin-degrading activity.

作者信息

Arregui A, Lee C M, Emson P C, Iversen L L

出版信息

Eur J Pharmacol. 1979 Oct 26;59(1-2):141-4. doi: 10.1016/0014-2999(79)90037-2.

Abstract

Angiotensin-converting enzyme and enkephalin-degrading enzyme activities were solubilized and purified from a particulate fraction of human diencephalon. Converting enzyme activity and enkephalin-degrading activity elute in different fractions following ion exchange chromatography on DEAE-cellulose, suggesting that they are different enzymes. Both enzymes were purified further by ion exchange chromatography on hydroxylapatite and by gel filtration on Sephadex G-200. The purified enzymes had markedly different sensitivities to known inhibitors of angiotensin-converting enzyme. The data do not support the hypothesis that angiotensin-converting enzyme and enkephalin degrading-enzyme are identical.

摘要

血管紧张素转换酶和脑啡肽降解酶活性是从人丘脑的微粒部分中溶解并纯化出来的。在DEAE - 纤维素上进行离子交换色谱后，转换酶活性和脑啡肽降解活性在不同的馏分中洗脱，这表明它们是不同的酶。两种酶通过在羟基磷灰石上进行离子交换色谱和在Sephadex G - 200上进行凝胶过滤进一步纯化。纯化后的酶对已知的血管紧张素转换酶抑制剂具有明显不同的敏感性。这些数据不支持血管紧张素转换酶和脑啡肽降解酶是同一酶的假设。