Department of Internal Diseases and Allergology, Medical University of Wroclaw, Wroclaw, Poland.
J Investig Allergol Clin Immunol. 2009;19(6):465-73.
The aim of our study was to determine the diagnostic usefulness of a newly developed basophil activation test (BAT) in patients allergic to Dermatophagoides pteronyssinus and pollens. We also analyzed the influence of cetirizine on CD63 upregulation. This popular antihistamine strongly inhibits skin tests, but its impact on BAT sensitivity remains unknown and deserves at least preliminary determination.
The study sample comprised 22 patients allergic to house dust mite and pollens and 19 healthy controls. All participants underwent skin prick testing and the newly developed flow-cytometric basophil activation test. The protocol for allergen-induced basophil CD63 upregulation consisted of whole blood samples that were processed and stained with anti-CCR3/CD63 antibodies added to the buffer at the beginning of stimulation. Skin prick tests and BAT were performed twice--before and 2 hours after ingestion of 10 mg of cetirizine.
The new BAT is characterized by its short processing time, easy basophil gating, and strong CD63 upregulation with very high sensitivity and excellent specificity. Our results suggest that allergen-induced CD63 upregulation by higher doses of allergens is not inhibited 2 hours after administration of cetirizine (unlike skin prick tests).
The BAT is a very useful and precise method for the diagnosis of allergy to aeroallergens. It is not influenced by cetirizine.
我们研究的目的是确定新开发的嗜碱性粒细胞活化试验（BAT）在对屋尘螨和花粉过敏的患者中的诊断价值。我们还分析了西替利嗪对 CD63 上调的影响。这种广受欢迎的抗组胺药强烈抑制皮肤测试，但它对 BAT 敏感性的影响尚不清楚，值得至少初步确定。
研究样本包括 22 名对屋尘螨和花粉过敏的患者和 19 名健康对照者。所有参与者均接受皮肤点刺试验和新开发的流式细胞术嗜碱性粒细胞活化试验。过敏原诱导的嗜碱性粒细胞 CD63 上调方案包括全血样本，这些样本在刺激开始时用添加到缓冲液中的抗 CCR3/CD63 抗体进行处理和染色。在服用 10mg 西替利嗪之前和之后 2 小时进行皮肤点刺试验和 BAT。
新的 BAT 具有处理时间短、易于嗜碱性粒细胞门控、CD63 上调强烈等特点，具有很高的灵敏度和出色的特异性。我们的结果表明，过敏原诱导的 CD63 上调在服用西替利嗪 2 小时后不会被更高剂量的过敏原抑制（与皮肤点刺试验不同）。
BAT 是一种非常有用和精确的诊断空气过敏原过敏的方法。它不受西替利嗪的影响。
