Ashraf M, Akram N A, Foolad M R
Department of Botany, University of Agriculture, Faisalabad, Pakistan.
Methods Mol Biol. 2012;913:305-33. doi: 10.1007/978-1-61779-986-0_21.
Marker-assisted selection (MAS) is the process of using morphological, biochemical, or DNA markers as indirect selection criteria for selecting agriculturally important traits in crop breeding. This process is used to improve the effectiveness or efficiency of selection for the traits of interest in breeding programs. The significance of MAS as a tool for crop improvement has been extensively investigated in different crop -species and for different traits. The use of MAS for manipulating simple/qualitative traits is straightforward and has been well reported. However, MAS for the improvement of complex/polygenic traits, including plant tolerance/resistance to abiotic stresses, is more complicated, although its usefulness has been recognized. With the recent advances in marker technology, including high-throughput genotyping of plants, together with the development of nested association mapping populations, it is expected that the utility of MAS for breeding for stress tolerance traits will increase. In this chapter, we describe the basic procedure for using MAS in crop breeding for salt tolerance.
标记辅助选择（MAS）是指利用形态学、生物化学或DNA标记作为间接选择标准，在作物育种中选择具有农业重要性的性状的过程。该过程用于提高育种计划中目标性状选择的有效性或效率。作为一种作物改良工具，MAS的重要性已在不同作物品种和不同性状上得到广泛研究。利用MAS操纵简单/质量性状的方法简单明了，已有大量报道。然而，利用MAS改良复杂/多基因性状，包括植物对非生物胁迫的耐受性/抗性，虽然其有用性已得到认可，但更为复杂。随着标记技术的最新进展，包括植物的高通量基因分型，以及嵌套关联作图群体的发展，预计MAS在耐胁迫性状育种中的应用将增加。在本章中，我们描述了在作物耐盐性育种中使用MAS的基本程序。
