Li Fayuan, Liu Xun, Wu Shengyan, Luo Qingyun, Yu Bingjun
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
College of Horticulture Sciences, Nanjing Agricultural University, Nanjing, China.
PeerJ. 2019 Feb 19;7:e6483. doi: 10.7717/peerj.6483. eCollection 2019.
cultivars Lee68, Nannong 1138-2, and Nannong 8831 were used as the female parents, and hybrid lines (F) 4,111, 4,076 (N23674 × BB52), 3,060 (Lee68 × N23227), and 185 (Jackson × BB52) that selected for salt tolerance generation by generation from the cross combination of and were used as the male parents, 11 (A-K) backcrosses or three-way crosses were designed and 213 single hybrids were harvested. The optimized soybean simple sequence repeat (SSR)-polymerase chain reaction (PCR) system was used to analyze the SSR polymorphism of above parental lines and get the parental co-dominant SSR markers for hybrid identification, and in which 30 true hybrids were gained. The true hybrids (G1, G3, G9, G12, G13, G16) of G cross combination were chosen as the representative for the salt tolerance test, and the results showed that, as exposed to salt stress, the seedlings of G9 line displayed higher salt tolerant coefficient, relative growth rate, and dry matter accumulation, when compared with their female parent Nannong 1138-2, and even performed equally strong salt tolerance as the male parent 3,060. It provides a feasible method of the combination of molecular SSR markers and simple physiological parameters to identify the true hybrids of and , and to innovate the salt-tolerant soybean germplasms.
以李68、南农1138 - 2和南农8831品种作为母本，以及通过 与 的杂交组合逐代筛选出的耐盐性杂交系（F）4111、4076（N23674×BB52）、3060（李68×N23227）和185（杰克逊×BB52）作为父本，设计了11个（A - K）回交或三交组合，并收获了213个单交种。采用优化的大豆简单序列重复（SSR）-聚合酶链反应（PCR）体系分析上述亲本系的SSR多态性，获得用于杂交鉴定的亲本共显性SSR标记，从中得到30个真杂种。选择G杂交组合的真杂种（G1、G3、G9、G12、G13、G16）作为耐盐性测试的代表，结果表明，在盐胁迫下，G9系幼苗与母本南农1138 - 2相比，表现出更高的耐盐系数、相对生长速率和干物质积累，甚至与父本3060表现出同样强的耐盐性。这为结合分子SSR标记和简单生理参数鉴定 和 的真杂种以及创新耐盐大豆种质提供了一种可行的方法。
