Knöpfel lab for Neuronal Circuit Dynamics, RIKEN Brain Science Institute , 2-1 Hirosawa, Wako City, Saitama, 351-0198 Japan.
ACS Chem Neurosci. 2012 Aug 15;3(8):585-92. doi: 10.1021/cn300041b. Epub 2012 Jun 6.
Fluorescent membrane voltage indicators that enable optical imaging of neuronal circuit operations in the living mammalian brain are powerful tools for biology and particularly neuroscience. Classical voltage-sensitive dyes, typically low molecular-weight organic compounds, have been in widespread use for decades but are limited by issues related to optical noise, the lack of generally applicable procedures that enable staining of specific cell populations, and difficulties in performing imaging experiments over days and weeks. Genetically encoded voltage indicators (GEVIs) represent a newer alternative that overcomes several of the limitations inherent to classical voltage-sensitive dyes. We critically review the fundamental concepts of this approach, the variety of available probes and their state of development.
荧光膜电压指示剂使在活体哺乳动物大脑中光学成像神经元回路操作成为可能，是生物学，尤其是神经科学的有力工具。经典的电压敏感染料，通常是低分子量的有机化合物，已经广泛使用了几十年，但受到与光学噪声相关的问题的限制，缺乏能够对特定细胞群体进行染色的普遍适用的程序，并且在数天和数周内进行成像实验存在困难。遗传编码的电压指示剂 (GEVI) 代表了一种新的替代方法，克服了经典电压敏感染料固有的一些局限性。我们批判性地回顾了这种方法的基本概念、可用探针的种类及其发展状况。
