Wu Yi-Hang, Hao Bing-Jie, Shen E, Meng Qing-Li, Hu Ming-Hui, Zhao Yu
Department of Pharmacy, College of Life Sciences, China Jiliang University, 310018 Hangzhou, China.
Sci Pharm. 2012 Apr-Jun;80(2):447-56. doi: 10.3797/scipharm.1108-16. Epub 2012 Feb 8.
Laggera alata extract (LAE) was quantitatively analyzed, and its principle components isochlorogenic acids were isolated and authenticated. Protective properties of LAE were studied using a d-galactosamine (d-GalN)-induced injury model in neonatal rat hepatocytes and a d-GalN-induced acute liver damage model in mice. Meanwhile, the effect of isochlorogenic acids derived from LAE on d-GalN-induced hepatocyte injury were also measured in vitro. LAE at concentrations of 10-100 μg/ml significantly reduced cellular leakage of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and improved cell viability. The isochlorogenic acids (4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 3,4-O-dicaffeoylquinic acid) at concentrations of 1-100 μg/ml also remarkably improved viability of hepatocytes. The oral treatment of LAE at doses of 50, 100 and 200 mg/kg markedly reduced the serum AST and ALT activity of mice and resulted in significant recovery of hepatocytes in liver sections.
对六棱菊提取物（LAE）进行了定量分析，并分离鉴定了其主要成分异绿原酸。使用新生大鼠肝细胞中d - 半乳糖胺（d - GalN）诱导的损伤模型和小鼠中d - GalN诱导的急性肝损伤模型研究了LAE的保护特性。同时，还在体外测定了LAE衍生的异绿原酸对d - GalN诱导的肝细胞损伤的影响。浓度为10 - 100μg/ml的LAE显著降低了天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的细胞泄漏，并提高了细胞活力。浓度为1 - 100μg/ml的异绿原酸（4,5 - O - 二咖啡酰奎宁酸、3,5 - O - 二咖啡酰奎宁酸和3,4 - O - 二咖啡酰奎宁酸）也显著提高了肝细胞的活力。以50、100和200mg/kg的剂量口服LAE可显著降低小鼠血清AST和ALT活性，并使肝切片中的肝细胞显著恢复。
