Laboratoire de Parasitologie-Mycologie code 99UR/08-05, Faculté de Pharmacie, Département de Biologie Clinique B, 1 Rue Avicenne, 5000 Monastir, Tunisie.
J Med Entomol. 2012 Jul;49(4):787-93. doi: 10.1603/me11110.
Morphological and molecular characterization of Phlebotomus (Larroussius) perniciosus Newstead and Phlebotomus (L.) longicuspis Nitzulescu in Tunisia is reported. Different localities in central and southern Tunisia were sampled. Sand flies were collected by sticky-paper traps and Center for Disease Control traps. For morphological study of males, the copulatory valves (aedeagi) were examined and the number of coxite hairs was recorded. For molecular analysis, the mitochondrial cytochrome b gene and the cytochrome c oxidase I gene were sequenced to investigate the population structure of P. perniciosus and P. longicuspis. The majority of P. perniciosus samples from southern and some specimens from central Tunisia showed single-pointed aedeagi curved at their apicies that were indistinguishable from the P. longicuspis aedeagi and appeared similar to the atypical morph of P. perniciosus, previously described in northern Morocco. The current study gives evidence of a wide distribution of atypical morphs of P. perniciosus in southern and central Tunisia.
报道了突尼斯的白蛉属（拉里乌斯）蚋和白蛉属（L.）长刺亚种的形态和分子特征。在突尼斯中部和南部的不同地点进行了采样。用粘性纸陷阱和疾病控制中心陷阱收集了沙蝇。为了研究雄性的形态，检查了交配瓣（阳茎）并记录了肛毛的数量。为了进行分子分析，对线粒体细胞色素 b 基因和细胞色素 c 氧化酶 I 基因进行了测序，以调查白蛉属蚋和白蛉属长刺亚种的种群结构。来自南部和一些来自中部突尼斯的大多数白蛉属蚋样本显示出尖端弯曲的单尖阳茎，与白蛉属长刺亚种的阳茎无法区分，与先前在摩洛哥北部描述的白蛉属蚋的非典型形态相似。本研究证明了突尼斯南部和中部存在广泛分布的白蛉属蚋的非典型形态。
