Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, South Korea.
J Agric Food Chem. 2012 Sep 12;60(36):9120-9. doi: 10.1021/jf3022018. Epub 2012 Aug 28.
Ecklonia stolonifera is a brown alga that was shown to have antioxidant, anti-inflammatory, tyrosinase inhibitory, and chemopreventive activities. However, the molecular mechanisms underlying its anti-inflammatory activity remain unclear. In this study, we investigated the molecular mechanism of the anti-inflammatory action of E. stolonifera ethanolic extracts (ESE) using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESE inhibited LPS-induced nitric oxide (IC(50) = 72 ± 1.9 μg/mL) and prostaglandin E(2) (IC(50) = 98 ± 5.3 μg/mL) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. ESE also reduced the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. LPS-induced nuclear factor-κB (NF-κB) transcriptional activity and NF-κB translocation into the nucleus were significantly inhibited by ESE treatment through the prevention of the degradation of inhibitor κB-α. Moreover, ESE inhibited the activation of Akt, ERK, JNK1/2, and p38 MAPK in LPS-stimulated RAW 264.7 cells. The main components with anti-inflammatory activity in ESE were identified as phlorofucofuroeckol A and B based on the inhibition of NO production. Our results indicate that ESE can be considered as a potential source of therapeutic agents for inflammatory diseases.
裙带菜是一种褐藻，具有抗氧化、抗炎、抑制酪氨酸酶和化学预防作用。然而，其抗炎活性的分子机制尚不清楚。在这项研究中，我们使用脂多糖（LPS）刺激的 RAW 264.7 细胞研究了裙带菜乙醇提取物（ESE）抗炎作用的分子机制。ESE 以剂量依赖性方式抑制 LPS 诱导的一氧化氮（IC50 = 72 ± 1.9 μg/mL）和前列腺素 E2（IC50 = 98 ± 5.3 μg/mL）的产生，并抑制 RAW 264.7 细胞中诱导型一氧化氮合酶和环氧化酶-2 的表达。ESE 还降低了 LPS 刺激的 RAW 264.7 细胞中促炎细胞因子的产生。ESE 通过阻止抑制剂 κB-α 的降解，显著抑制 LPS 诱导的核因子-κB（NF-κB）转录活性和 NF-κB 向核内易位。此外，ESE 抑制了 LPS 刺激的 RAW 264.7 细胞中 Akt、ERK、JNK1/2 和 p38 MAPK 的激活。根据 NO 产生的抑制作用，确定 ESE 中具有抗炎活性的主要成分是 phlorofucofuroeckol A 和 B。我们的结果表明，ESE 可以被认为是治疗炎症性疾病的潜在药物来源。
