Centro para el Desarrollo de la Nanociencia y Nanotecnología, Universidad de Santiago, Santiago, Chile.
Reprod Biol Endocrinol. 2012 Aug 16;10:56. doi: 10.1186/1477-7827-10-56.
One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.
In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.
Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.
Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.
女性生殖道的一个独特特征是在整个月经周期中观察到的广泛组织重塑。细胞外基质的多个成分参与了这种组织重建；然而，参与的单个成分尚未确定。
在本研究中，通过 PCR 阵列、免疫细胞化学、凝胶电泳和生物信息学研究了整个月经周期输卵管 (FT) 中外细胞基质蛋白和选定基质金属蛋白酶 (MMP) 活性的表达。
在所分析的 84 个基因中，有 83 个在 FT 中至少在月经周期的一个阶段表达。与卵泡期相比，我们观察到在排卵前阶段 ADAMTS1、ADAMTS13、COL7A1、MMP3、MMP9、PECAM1 和 THBS3 的表达显着增加（>/=2 倍）。同时，我们观察到黄体期与排卵前阶段相比，COL7A1、ICAM1、ITGA8、MMP16、MMP9、CLEC3B、SELE 和 TIMP2 的表达显着降低（>/=2 倍）。免疫细胞化学显示 MMP-3 和 MMP-9 在月经周期的所有阶段都定位于内输卵管。明胶酶谱检测到非周期性蛋白酶活性。
几种细胞外基质成分在月经周期中呈周期性调节，提示可能存在类固醇调节作用以及在组织重塑和 FT 功能中的作用。
