Monti J P, Sarrazin M, Gallice P, Crevat A, Baz M, Murisasco A, Elsen R
Laboratory of Biophysics, Faculty de Pharmacy, Marseille, France.
Int J Artif Organs. 1990 Dec;13(12):799-802.
Changes in intra-erythrocytic pH values over time, during and after bicarbonate hemodialysis, were studied with 31P Nuclear Magnetic Resonance. Simultaneously, pH values of whole blood were obtained by a gazometric method. A two-compartment model appeared to be the simplest kinetic model to explain the shifts in proton concentrations in extra- and intra-cellular media. Non-linear regression was used to determine exchange constant values. There was a very good correlation between the experimental and calculated proton concentrations. This model can describe all patients but individual experimental constants must be determined. Under these conditions a single blood pH determination before dialysis will permit determination of the initial intra-erythrocytic pH and monitoring of intra-erythrocytic pH during hemodialysis.
利用31P核磁共振技术研究了在碳酸氢盐血液透析期间及之后红细胞内pH值随时间的变化。同时，通过气体测定法获得全血的pH值。两室模型似乎是解释细胞外和细胞内介质中质子浓度变化的最简单动力学模型。使用非线性回归来确定交换常数的值。实验测定的质子浓度与计算得到的质子浓度之间具有很好的相关性。该模型可以描述所有患者，但必须确定个体实验常数。在这些条件下，透析前单次测定血液pH值将有助于确定初始红细胞内pH值，并监测血液透析期间的红细胞内pH值。
