Grup de Sensors i Biosensors, Departament de Química, Facultat de Ciències, Edifici C-nord, UniversitatAutònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.
Nanoscale. 2012 Sep 28;4(19):5917-23. doi: 10.1039/c2nr31257c. Epub 2012 Aug 17.
The combination of optimized and passivated Field Effect Transistors (FETs) based on carbon nanotubes (CNTs) together with the appropriate choice and immobilization strategy of aptamer receptors and buffer concentration have allowed the highly sensitive and real time biorecognition of proteins in a liquid-gated configuration. Specifically we have followed the biorecognition process of thrombin by its specific aptamer. The aptamer modified device is sensitive enough to capture a change in the electronic detection mechanism, one operating at low protein concentrations and the other in a higher target concentration range. The high sensitivity of the device is also sustained by the very low detection limits achieved (20 pM) and their high selectivity when other target proteins are used. Moreover, the experimental results have allowed us to quantify the equilibrium constant of the protein-aptamer binding and confirm its high affinity by using the Langmuir equation.
基于碳纳米管（CNTs）的优化和钝化场效应晶体管（FET）的组合，以及适当地选择和固定化适体受体和缓冲液浓度的策略，使得在液栅配置下能够高度敏感和实时地进行蛋白质的生物识别。具体来说，我们跟踪了其特定适体的凝血酶的生物识别过程。该适体修饰的器件具有足够的灵敏度，可以捕捉到电子检测机制的变化，一种在低蛋白质浓度下工作，另一种在更高的目标浓度范围内工作。该器件的高灵敏度还得益于实现的非常低的检测限（20 pM）及其在使用其他靶蛋白时的高选择性。此外，实验结果还允许我们使用朗缪尔方程来量化蛋白质-适体结合的平衡常数，并证实其高亲和力。
