用于碳纳米管场效应晶体管适配体传感器的双链和四链折叠结构腺苷适配体的比较
Comparison of Duplex and Quadruplex Folding Structure Adenosine Aptamers for Carbon Nanotube Field Effect Transistor Aptasensors.
作者信息
Nguyen Hong Phan T, Murugathas Thanihaichelvan, Plank Natalie O V
机构信息
School of Chemical and Physical Sciences, Victoria University of Wellington, Wellington 6021, New Zealand.
The MacDiarmid Institute for Advanced Materials and Nanotechnolgy, Victoria University of Wellington, Wellington 6021, New Zealand.
出版信息
Nanomaterials (Basel). 2021 Sep 2;11(9):2280. doi: 10.3390/nano11092280.
Carbon nanotube field effect transistor (CNT FET) aptasensors have been investigated for the detection of adenosine using two different aptamer sequences, a 35-mer and a 27-mer. We found limits of detection for adenosine of 100 pM and 320 nM for the 35-mer and 27-mer aptamers, with dissociation constants of 1.2 nM and 160 nM, respectively. Upon analyte recognition the 35-mer adenosine aptamer adopts a compact G-quadruplex structure while the 27-mer adenosine aptamer changes to a folded duplex. Using the CNT FET aptasensor platform adenosine could be detected with high sensitivity over the range of 100 pM to 10 µM, highlighting the suitability of the CNT FET aptasensor platform for high performance adenosine detection. The aptamer restructuring format is critical for high sensitivity with the G-quadraplex aptasensor having a 130-fold smaller dissociation constant than the duplex forming aptasensor.
碳纳米管场效应晶体管（CNT FET）适体传感器已被用于研究使用两种不同的适体序列（一种35聚体和一种27聚体）检测腺苷。我们发现，对于35聚体和27聚体适体，腺苷的检测限分别为100 pM和320 nM，解离常数分别为1.2 nM和160 nM。在识别分析物时，35聚体腺苷适体采用紧密的G-四链体结构，而27聚体腺苷适体则转变为折叠的双链体。使用CNT FET适体传感器平台，在100 pM至10 µM的范围内可以高灵敏度地检测腺苷，突出了CNT FET适体传感器平台用于高性能腺苷检测的适用性。适体的重组形式对于高灵敏度至关重要，G-四链体适体传感器的解离常数比形成双链体的适体传感器小130倍。
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