Curiale M S, Klatt M J, Gehle W D, Chandonnet H E
Silliker Laboratories, Inc., Chicago Heights, IL 60411.
J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):961-8.
Two modified fluorescent enzyme immunoassays for the detection of Salmonella in food have been developed. Both of the new procedures, which substitute a colorimetric substrate for the fluorescent substrate and in which results are read visually or with a photometer, are modifications of AOAC method 989.15. The visually read procedure uses the same antibody-coated wells as in method 989.15. The colorimetric end point of the assay is determined by comparing the solution color to a color chart. The assay result may also be read in a photometer, if the solution is first transferred to a transparent microtiter well. The second procedure designed to be read in a photometer substitutes clear, antibody-coated wells for those used in the fluorescent assay. The colorimetric assays employ identical monoclonal antibodies for capture and detection of Salmonella as used in the fluorescent assay. In this comparative study, the performance of each new assay was consistent with the performance of method 989.15. These methods have been adopted official first action by AOAC as alternative methods for the detection of Salmonella in foods.
已开发出两种用于检测食品中沙门氏菌的改良荧光酶免疫测定法。这两种新方法均采用比色底物替代荧光底物，结果可通过目视或用光度计读取，它们都是对美国官方分析化学师协会（AOAC）方法989.15的改良。目视读取法使用与方法989.15相同的包被抗体孔。通过将溶液颜色与比色卡进行比较来确定测定的比色终点。如果先将溶液转移到透明微量滴定孔中，也可以用光度计读取测定结果。第二种设计用于光度计读取的方法用透明的包被抗体孔替代了荧光测定中使用的孔。比色测定法使用与荧光测定法相同的单克隆抗体来捕获和检测沙门氏菌。在这项比较研究中，每种新方法的性能与方法989.15的性能一致。这些方法已被AOAC正式首次采用为食品中沙门氏菌检测的替代方法。
