Fantasia L D, Schrade J P, Yager J F, Debler D
J Assoc Off Anal Chem. 1975 Jul;58(4):828-44.
In preliminary studies, several commercial polyvalent fluorescent antibody (FA) preparations were evaluated for specificity and crossreactivity and an FA method was developed for the screening of Salmonella in products. Approximately 4000 product samples were tested by the FA method and the results were compared to those from the official final action AOAC method, 46.013-46.026. Only 4 FA false-negatives were found for a total of 619 confirmed positive Salmonella samples. The FA false-positive rate was 7%. The method was then subjected to a 2-phase collaborative study. In Phase I, 22 analysts tested 5 inoculated and 5 uninoculated samples of dried milk. In Phase II, 5 naturally contaminated and 5 presumably uncontaminated foods were analyzed. The study was designed to compare results from 11 analysts experienced in FA methodology with those from 11 analysts with little or no experience. Selenite cystine (SC) and tetrathionate (TT) broths were used for enrichment and both were inoculated into SC for post-enrichment. All 4 combinations (SC, TT, SC-SC, and TT-SC) were used with the FA method to determine the best technique. Results were compared to the analysis with TT and SC by the AOAC culture method. In all studies, FA analysis with SC-SC gave the highest correlation with the AOAC method. In a total of 200 samples, the experienced group found 125 AOAC positives and 127 FA positives; no FA false-negatives and only 2 false-positives were reported. The inexperienced group reported 9 FA false-negatives and 5 FA false-positives. All false-negatives occurred in only 3 of the inexperienced laboratories. These studies showed that enrichment and post-enrichment in SC gave the best FA results and that training in FA methodology is required for correlation with existing AOAC methodology. The FA method for the detection of Salmonella has been adopted as official first action.
在初步研究中，对几种市售多价荧光抗体（FA）制剂的特异性和交叉反应性进行了评估，并开发了一种用于筛查产品中沙门氏菌的FA方法。采用FA方法检测了约4000份产品样本，并将结果与官方最终采用的AOAC方法（46.013 - 46.026）的结果进行比较。在总共619份经确认的沙门氏菌阳性样本中，仅发现4例假阴性FA结果。FA假阳性率为7%。然后该方法进行了两阶段的协作研究。在第一阶段，22名分析人员检测了5份接种和5份未接种的奶粉样本。在第二阶段，分析了5份自然污染和5份可能未受污染的食品。该研究旨在比较11名有FA方法经验的分析人员与11名经验很少或没有经验的分析人员的结果。使用亚硒酸盐胱氨酸（SC）肉汤和四硫磺酸盐（TT）肉汤进行增菌，并将两者接种到SC肉汤中进行后增菌。所有4种组合（SC、TT、SC - SC和TT - SC）都与FA方法一起用于确定最佳技术。将结果与AOAC培养方法对TT和SC的分析结果进行比较。在所有研究中，SC - SC的FA分析与AOAC方法的相关性最高。在总共200份样本中，有经验的小组发现125份AOAC阳性和127份FA阳性；未报告FA假阴性，仅报告了2例假阳性。经验不足的小组报告了9例假阴性FA结果和5例假阳性FA结果。所有假阴性仅发生在3个经验不足的实验室中。这些研究表明，在SC中进行增菌和后增菌可获得最佳的FA结果，并且为了与现有的AOAC方法相关联，需要对FA方法进行培训。检测沙门氏菌的FA方法已被采用为官方首次采用方法。
