Bhasin Sheetal, Modi H A
Department of Biosciences, Maharaja Ranjit Singh College of Professional Sciences, Madhya Pradesh, Indore 452001, India.
Biotechnol Res Int. 2012;2012:874152. doi: 10.1155/2012/874152. Epub 2012 Jul 26.
The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of numerous enzymes including glucose isomerase. An array of 75 isolates was screened for the production of glucose isomerase. The isolate Streptomyces sp. SB-P1 was found to produce maximum amount of extracellular GI. Sucrose and raffinose among pure carbon sources and corn cob and wheat husk among crude agro residues were found to yield high enzyme titers. Potassium nitrate among pure nitrogen sources and soy residues among crude sources gave maximum production. Quantitative effect of carbon, nitrogen, and inducer on GI was also determined. Plackett-Burman design was used to study the effect of different medium ingredients. Sucrose and xylose as carbon sources and peptone and soy residues as nitrogen sources proved to be beneficial for GI production.
培养基成分的组合对微生物中运行的代谢途径具有深远影响，这些代谢途径调节着众多代谢产物的产生。葡萄糖异构酶（GI）是一种在市场上具有巨大潜力的酶，它能将葡萄糖异构化为果糖。GI被广泛用于生产高果糖玉米糖浆（HFCS）。HFCS在食品和制药行业中用作甜味剂。链霉菌是包括葡萄糖异构酶在内的众多酶的著名生产者。对75株分离株进行了葡萄糖异构酶生产的筛选。发现链霉菌属SB - P1分离株产生的细胞外GI量最大。在纯碳源中，蔗糖和棉子糖以及在粗农业残留物中玉米芯和麦麸被发现能产生高酶活性。在纯氮源中硝酸钾和在粗源中大豆残渣产生的产量最高。还确定了碳、氮和诱导剂对GI的定量影响。采用Plackett - Burman设计研究不同培养基成分的影响。结果表明，蔗糖和木糖作为碳源以及蛋白胨和大豆残渣作为氮源有利于GI的生产。
