Rengasamy Sathya, Subramanian Mohan Raj, Perumal Varalakshmi, Ganeshan Shakambari, Al Khulaifi Manal M, Al-Shwaiman Hind A, Elgorban Abdallah M, Syed Asad, Thangaprakasam Ushadevi
Centre for Research and Development, PRIST University, Tamil Nadu 613-403, India.
Department of Molecular Biology, Madurai Kamaraj University, Madurai 625-021, India.
Saudi J Biol Sci. 2020 Apr;27(4):1117-1123. doi: 10.1016/j.sjbs.2019.12.024. Epub 2019 Dec 23.
Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. are well-known producers of various industrially valuable enzymes, including Glucose isomerase. Currently, recombinant strains have been available for the production of various enzymes, but it has limitation in the large scale production. Therefore, identifying effective strains have emerged. The current study, the novel RSU26 was isolated from a marine source and optimized its potential to produce glucose isomerase at different physical and chemical conditions. The optimum pH and temperature for GI and biomass production were 7.5 and 35 °C, respectively at 96 h. Characterization study revealed that the approximate molar mass of GI was 43 kDa for monomeric and 170 kDa for tetrameric forms. Kinetic behavior exhibits , and values for the conversion of fructose to glucose conversion were 48.8 mM and 2.54 U mg at 50 °C and glucose to fructose were 29.4 mM and 2.38 U mg at 65 °C protein, respectively. Therefore, the present study suggested that the wild-type RSU26 has strong potential to produce glucose isomerase for various industrial applications.
葡萄糖异构酶（GI）是一种在世界市场上具有很高潜力的酶。GI在高果糖玉米糖浆生产（HFCS）中起着重要作用。HFCS被用作食品和制药行业的甜味剂。 是包括葡萄糖异构酶在内的各种具有工业价值酶的知名生产商。目前，重组菌株已可用于生产各种酶，但在大规模生产方面存在局限性。因此，鉴定有效的 菌株应运而生。在当前的研究中，新型RSU26从海洋来源中分离出来，并在不同的物理和化学条件下优化了其产生葡萄糖异构酶的潜力。在96小时时，GI和生物量生产的最佳pH值和温度分别为7.5和35°C。表征研究表明，GI的单体形式的近似摩尔质量为43 kDa，四聚体形式为170 kDa。动力学行为显示，在50°C时，果糖转化为葡萄糖的 、 值分别为48.8 mM和2.54 U mg，在65°C时，葡萄糖转化为果糖的 、 值分别为29.4 mM和2.38 U mg蛋白质。因此，本研究表明野生型RSU26具有为各种工业应用生产葡萄糖异构酶的强大潜力。
