Purification and kinetic behavior of glucose isomerase from RSU26.

Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. are well-known producers of various industrially valuable enzymes, including Glucose isomerase. Currently, recombinant strains have been available for the production of various enzymes, but it has limitation in the large scale production. Therefore, identifying effective strains have emerged. The current study, the novel RSU26 was isolated from a marine source and optimized its potential to produce glucose isomerase at different physical and chemical conditions. The optimum pH and temperature for GI and biomass production were 7.5 and 35 °C, respectively at 96 h. Characterization study revealed that the approximate molar mass of GI was 43 kDa for monomeric and 170 kDa for tetrameric forms. Kinetic behavior exhibits , and values for the conversion of fructose to glucose conversion were 48.8 mM and 2.54 U mg at 50 °C and glucose to fructose were 29.4 mM and 2.38 U mg at 65 °C protein, respectively. Therefore, the present study suggested that the wild-type RSU26 has strong potential to produce glucose isomerase for various industrial applications.