Loki Anthony Loperito, Rajamohan T
Department of Biochemistry University of Kerala, Kariavattom, Thiruvananthapuram 695 581, India.
Indian J Biochem Biophys. 2003 Oct;40(5):354-7.
Hepatoprotective and antioxidant effects of tender coconut water (TCW) were investigated in carbon tetrachloride (CCl4)-intoxicated female rats. Liver damage was evidenced by the increased levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and decreased levels of serum proteins and by histopathological studies in CCl4-intoxicated rats. Increased lipid peroxidation was evidenced by elevated levels of thiobarbituric acid reactive substance (TBARS) viz, malondialdehyde (MDA), hydroperoxides (HP) and conjugated dienes (CD), and also by significant decrease in antioxidant enzymes activities, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx) and glutathione reductase (GR) and also reduced glutathione (GSH) content in liver. On the other hand, CCl4-intoxicated rats treated with TCW retained almost normal levels of these constituents. Decreased activities of antioxidant enzymes in CCl4-intoxicated rats and their reversal of antioxidant enzyme activities in TCW treated rats, shows the effectiveness of TCW in combating CCl4-induced oxidative stress. Hepatoprotective effect of TCW is also evidenced from the histopathological studies of liver, which did not show any fatty infiltration or necrosis, as observed in CCl4-intoxicated rats.
研究了嫩椰子水（TCW）对四氯化碳（CCl4）中毒雌性大鼠的肝脏保护和抗氧化作用。在CCl4中毒大鼠中，血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）水平升高，血清蛋白水平降低，以及组织病理学研究均证明了肝脏损伤。硫代巴比妥酸反应物质（TBARS）即丙二醛（MDA）、氢过氧化物（HP）和共轭二烯（CD）水平升高，以及抗氧化酶活性如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（Gpx）和谷胱甘肽还原酶（GR）显著降低，同时肝脏中谷胱甘肽（GSH）含量减少，均证明脂质过氧化增加。另一方面，用TCW处理的CCl4中毒大鼠这些成分的水平几乎保持正常。CCl4中毒大鼠抗氧化酶活性降低，而TCW处理大鼠抗氧化酶活性恢复，表明TCW在对抗CCl4诱导的氧化应激方面有效。肝脏组织病理学研究也证明了TCW的肝脏保护作用，与CCl4中毒大鼠不同，未显示任何脂肪浸润或坏死。
