叶水提取物及其与叶的组合对苯肼诱导的贫血大鼠基本生化参数的影响。

Effect of Aqueous Extract of Leaves and Its Combination with Leaves on Essential Biochemical Parameters of Phenylhydrazine-Induced Anemic Rats.

作者信息

Ezeigwe Obiajulu Christian, Nzekwe Favour Amarachi, Nworji Ogechukwu Frances, Ezennaya Chidinma Felicia, Iloanya Ebele Lauretta, Asogwa Kingsley Kelechi

机构信息

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

Department of Chemistry/Biochemistry and Molecular Biology, Faculty of Science, Alex Ekwueme Federal University, Ndufu-Alike Ikwo, Ebonyi State, Nigeria.

出版信息

J Exp Pharmacol. 2020 Jul 1;12:191-201. doi: 10.2147/JEP.S254003. eCollection 2020.

DOI:10.2147/JEP.S254003

PMID:32669880

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335891/

Abstract

BACKGROUND

Moraceae and Euphorbiaceae leaves have been used separately in traditional medical practice to treat different ailments, of which anemia is one. This study aims to evaluate the effect of and on hepatic, renal parameters and oxidative stress of phenylhydrazine-induced anemic rats.

METHODS

Thirty-five rats were randomized into seven groups (A-G) of five rats each. Groups A and B served as the normal control and anemic control, respectively, while Groups C, D, E, F, and G were treated with a standard drug (vitamin B), 200mg/kg bw. aqueous extract of , 400mg/kg b.w. aqueous extract of , 200mg/kg bw. of a combination of aqueous extract of and and 400mg/kg bw. of a combination of aqueous extract of and respectively. The biochemical analysis (liver and kidney function analysis, oxidative stress) was carried out using standard diagnostic techniques.

RESULTS

The results showed that there was significant decrease (<0.05) in the values obtained for Aspartate Transaminase (AST), alanine Transaminase (ALT), Alkaline Phosphatase (ALP), total bilirubin, urea, creatinine, potassium ion, Total Cholesterol (TCHOL), Low-density Lipoprotein (LDL-C), Triglycerides (TRIG), Very Low-density Lipoprotein (VLDL-C) and Malondialdehyde (MDA) and a significant increase (<0.05) in obtained values for High-density Lipoprotein (HDL) in all the extract-treated groups compared with the anemic-untreated. The values obtained for most of these biochemical parameters in the extract-treated groups were in the range of the normal control showing that the extract did not, in any way, alter the biochemical parameters. There was a significant increase (<0.05) in the glutathione peroxidase (Glut. Perox.) enzyme activity of the groups treated with the aqueous extract of and its combination with compared with the anemic-untreated.

CONCLUSION

These results suggest that the aqueous extracts of and leaves may promote liver function parameters, maintain normal serum electrolyte level and kidney function indices, stimulate reduction of "bad cholesterols" and increase "good cholesterol" and reduce oxidative stress.

摘要

背景

桑科和大戟科植物的叶子在传统医学实践中分别用于治疗不同疾病，贫血是其中之一。本研究旨在评估桑科和大戟科植物叶子对苯肼诱导的贫血大鼠肝脏、肾脏参数及氧化应激的影响。

方法

将35只大鼠随机分为7组（A - G），每组5只。A组和B组分别作为正常对照组和贫血对照组，而C组、D组、E组、F组和G组分别用标准药物（维生素B）、200mg/kg体重的桑科植物叶子水提取物、400mg/kg体重的大戟科植物叶子水提取物、200mg/kg体重的桑科植物叶子水提取物与大戟科植物叶子水提取物的组合以及400mg/kg体重的桑科植物叶子水提取物与大戟科植物叶子水提取物的组合进行处理。使用标准诊断技术进行生化分析（肝功能和肾功能分析、氧化应激）。

结果

结果显示，与未治疗的贫血组相比，所有提取物处理组的天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、总胆红素、尿素、肌酐、钾离子、总胆固醇（TCHOL）、低密度脂蛋白（LDL - C）、甘油三酯（TRIG）、极低密度脂蛋白（VLDL - C）和丙二醛（MDA）的值均显著降低（<0.05），而高密度脂蛋白（HDL）的值显著升高（<0.05）。提取物处理组中大多数这些生化参数的值在正常对照组范围内，表明提取物在任何方面均未改变生化参数。与未治疗的贫血组相比，用桑科植物叶子水提取物及其与大戟科植物叶子水提取物的组合处理的组中谷胱甘肽过氧化物酶（Glut. Perox.）酶活性显著增加（<0.05）。