使用 Lewis X 功能化磁性纳米颗粒检测和分离树突状细胞。
Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles.
机构信息
Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA.
出版信息
Biomacromolecules. 2012 Oct 8;13(10):3039-45. doi: 10.1021/bm3007506. Epub 2012 Sep 11.
Abstract
dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le(X)). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le(X) glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.
摘要
树突状细胞（DC）特异性细胞间黏附分子 3 抓取非整合素（DC-SIGN）是一种在 DC 上发现的受体，可识别具有甘露糖丰富或岩藻糖基化糖的抗原，包括 Lewis X（Le(X)）。在这里，我们报告了使用 Cu（I）催化的叠氮-炔环加成反应制备带有多价 Le(X)聚糖的磁性纳米粒子。所得的纳米粒子具有选择性和生物相容性，可用作高效的 DC 检测和富集工具。
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