College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, PR China.
Res Vet Sci. 2013 Feb;94(1):62-8. doi: 10.1016/j.rvsc.2012.07.025. Epub 2012 Aug 15.
Probiotics, including Bacillus spp. and Lactobacillus, are potential replacements for low dose in-feed antibiotics for pig. This study aimed to evaluate the effect of the co-administration of Bacillus subtilis RJGP16 and Lactobacillus salivarius B1 as potential probiotics to stimulate local immune responses. Thirty two newborn piglets were divided into four groups and orally administrated with different combination of probiotics (none; RJGP16; B1; RJGP16 and B1) at the age of 0, 7 and 11 days. We analysed the parameters of the mucosal immunity of piglets a week after weaning. Our results showed that the gene expression of interleukin (IL)-6 in the duodenum and ileum, porcine beta-defensins (pBD)-2 in the duodenum were significantly increased (p<0.01) with co-administration of the RJGP16 and B1. Also the expression and release of TLR-2 and the number of immunoglobulin (Ig) A producing cells were increased (p<0.01). The results demonstrate that the co-administration of the two bacteria stimulate a more intense mucosal immunity than the administration of each bacterium alone.
益生菌，包括芽孢杆菌属和乳杆菌属，是低剂量饲料抗生素在猪中的潜在替代品。本研究旨在评估枯草芽孢杆菌 RJGP16 和唾液乳杆菌 B1 联合作为潜在益生菌刺激局部免疫应答的效果。将 32 头新生仔猪分为四组，并在 0、7 和 11 日龄时分别口服不同组合的益生菌（无；RJGP16；B1；RJGP16 和 B1）。我们在断奶后一周分析了仔猪黏膜免疫的参数。结果表明，十二指肠和回肠中的白细胞介素（IL）-6 基因表达、十二指肠中的猪β防御素（pBD）-2 显著增加（p<0.01），与 RJGP16 和 B1 联合使用。TLR-2 的表达和释放以及分泌型免疫球蛋白（Ig）A 产生细胞的数量也增加（p<0.01）。结果表明，两种细菌的联合使用比单独使用每种细菌刺激更强的黏膜免疫应答。
