College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China.
Microb Pathog. 2024 Oct;195:106852. doi: 10.1016/j.micpath.2024.106852. Epub 2024 Aug 13.
The purpose of this study was to evaluate the ability of Bacillus subtilis JATP3 to stimulate immune response and improve intestinal health in piglets during the critical weaning period. Twelve 28-day-old weaned piglets were randomly divided into two groups. One group was fed a basal diet, while the other group was fed a basal diet supplemented with B. subtilis JATP3 (1 × 10 CFU/mL; 10 mL) for 28 days. The results revealed a significant increase in the intestinal villus gland ratio of weaned piglets following the inclusion of B. subtilis JATP3 (P < 0.05). Inclusion of a probiotic supplement improve the intestinal flora of jejunum and ileum of weaned piglets. Metabolomics analysis demonstrated a notable rise in citalopram levels in the jejunum and ileum, along with elevated levels of isobutyric acid and isocitric acid in the ileum. The results of correlation analysis show that indicated a positive correlation between citalopram and microbial changes. Furthermore, the probiotic-treated group exhibited a significant upregulation in the relative expression of Claudin, Zonula Occludens 1 (ZO-1), and Interleukin 10 (IL-10) in the jejunum and ileum, while displaying a noteworthy reduction in the relative expression of Interleukin 1β (IL-1β). Overall, these findings suggest that B. subtilis JATP3 can safeguard intestinal health by modulating the structure of the intestinal microbiota and their metabolites, wherein citalopram might be a key component contributing to the therapeutic effects of B. subtilis JATP3.
本研究旨在评估枯草芽孢杆菌 JATP3 在仔猪断奶关键期刺激免疫反应和改善肠道健康的能力。将 12 头 28 日龄断奶仔猪随机分为两组，一组饲喂基础日粮，另一组在基础日粮中添加枯草芽孢杆菌 JATP3（1×10CFU/mL；10mL）饲喂 28 天。结果表明，添加枯草芽孢杆菌 JATP3 可显著提高断奶仔猪的肠道绒毛腺比（P<0.05）。添加益生菌补充剂可改善断奶仔猪空肠和回肠的肠道菌群。代谢组学分析表明，在空肠和回肠中，西酞普兰水平显著升高，回肠中异丁酸和异柠檬酸水平升高。相关性分析结果表明，西酞普兰与微生物变化呈正相关。此外，益生菌处理组空肠和回肠中 Claudin、Zonula Occludens 1（ZO-1）和白细胞介素 10（IL-10）的相对表达显著上调，白细胞介素 1β（IL-1β）的相对表达显著下调。总之，这些发现表明，枯草芽孢杆菌 JATP3 通过调节肠道微生物群及其代谢物的结构来保护肠道健康，其中西酞普兰可能是枯草芽孢杆菌 JATP3 发挥治疗作用的关键成分。
