Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Urol. 2012 Oct;188(4):1260-4. doi: 10.1016/j.juro.2012.06.012. Epub 2012 Aug 16.
CLU is a disulfide linked, heterodimeric protein associated with the clearance of cellular debris and apoptosis. We assessed the association of seminal CLU gene expression with seminal variables in fertile and infertile men.
A total of 124 men were divided into healthy, fertile men with normozoospermia, and men with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia. History was obtained, and clinical examination and semen analysis were done. In semen we assessed sperm acrosin activity, sperm DNA fragmentation and seminal CLU gene expression.
CLU RNA and CLU protein gene expression were significantly increased in semen samples of infertile men with oligoasthenoteratozoospermia > asthenoteratozoospermia > asthenozoospermia compared with healthy, fertile controls. CLU gene expression significantly correlated negatively with sperm count, motility, acrosin activity index, linearity index and linear velocity, and significantly correlated positively with the percent of sperm abnormal forms and DNA fragmentation.
CLU gene expression was significantly increased in the semen samples of infertile men. It correlated negatively with sperm count, motility, acrosin activity, linearity index and linear velocity, and positively with the percent of sperm abnormal forms and DNA fragmentation.
CLU 是一种二硫键连接的异二聚体蛋白，与细胞碎片的清除和细胞凋亡有关。我们评估了精子 CLU 基因表达与生育力正常和不育男性精液变量的相关性。
共 124 名男性分为健康、生育力正常且精子参数正常的男性，以及弱精症、少弱畸形精子症和严重少弱畸形精子症的男性。采集病史，进行临床检查和精液分析。在精液中，我们评估了精子顶体酶活性、精子 DNA 碎片化和精液 CLU 基因表达。
与健康、生育力正常的对照组相比，严重少弱畸形精子症、弱精症-少弱畸形精子症和弱精症患者的精液样本中 CLU RNA 和 CLU 蛋白基因表达显著增加。CLU 基因表达与精子计数、活力、顶体酶活性指数、直线性指数和直线速度呈显著负相关，与精子畸形率和 DNA 碎片化呈显著正相关。
不育男性的精液样本中 CLU 基因表达显著增加。它与精子计数、活力、顶体酶活性、直线性指数和直线速度呈负相关，与精子畸形率和 DNA 碎片化呈正相关。
