Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, Punjab, India.
Plant Signal Behav. 2012 Oct 1;7(10):1321-9. doi: 10.4161/psb.21435. Epub 2012 Aug 20.
Whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleryrodidae), is a serious pest of black gram, (Vigna mungo (L.) Hepper), an important legume pulse crop grown in north India. This research investigated the potential role of selected plant oxidative enzymes in resistance/susceptibility to whitefly in nine black gram genotypes. Oxidative enzyme activity was estimated spectrophotometrically from leaf samples collected at 30 and 50 d after sowing (DAS) from whitefly infested and uninfested plants. The enzymes showed different activity levels at different times after the infestation. The results indicated that in general, whitefly infestation increased the activities of peroxidase and decreased the catalase activity. Resistant genotypes NDU 5-7 and KU 99-20 recorded higher peroxidase and catalase activities at 30 and 50 DAS under whitefly-stress conditions as compared with non-stressed plants. The results suggest that the enhanced activities of the enzymes may contribute to bioprotection of black gram plants against B. tabaci infestation. The potential mechanisms to explain the correlation of resistance to whitefly in black gram genotypes with higher activities of oxidative enzymes are also discussed.
烟粉虱，Bemisia tabaci（Gennadius）（半翅目：粉虱科），是印度北部种植的重要豆科食用豆类作物——绿豆（Vigna mungo（L.）Hepper）的严重害虫。本研究调查了 9 个绿豆品种对烟粉虱的抗性/敏感性中选择的植物氧化酶的潜在作用。在接种烟粉虱和未接种烟粉虱的植株上，分别在播种后 30 和 50 天（DAS）从叶片样本中通过分光光度法估算氧化酶活性。在受侵染后不同时间，酶表现出不同的活性水平。结果表明，一般来说，烟粉虱侵染会增加过氧化物酶的活性，降低过氧化氢酶的活性。与非胁迫植株相比，在烟粉虱胁迫条件下，抗性基因型 NDU 5-7 和 KU 99-20 在 30 和 50 DAS 时过氧化物酶和过氧化氢酶的活性更高。结果表明，这些酶的活性增强可能有助于绿豆植物对 B. tabaci 侵染的生物保护。还讨论了解释绿豆基因型对烟粉虱抗性与氧化酶活性较高之间相关性的潜在机制。
