Burliński Piotr Józef, Czujkowska Agnieszka, Arciszewski Marcin B, Całka Jarosław
University of Warmia and Mazury Department of Clinical Physiology, Faculty of Veterinary Medicine Oczapowskiego 13 10-719 Olsztyn Poland.
Acta Vet Hung. 2012 Sep;60(3):383-93. doi: 10.1556/AVet.2012.033.
Both resiniferatoxin (RTX) and tetrodotoxin (TTX) have been reported to be effective in several clinical trials aiming to cure urinary bladder dysfunction. The goal of this experiment was to study the effect of intravesical administration of RTX and TTX on the chemical coding of paracervical ganglion (PCG) neurons that supply the urinary bladder in pigs. The vasoactive intestinal peptide (VIP) and the opioid family member Leu5-enkephalin (LENK) are both known for their regulatory effects in the function of the porcine genitourinary tract. The PCG neurons innervating the urinary bladder were identified by application of the retrograde tracer Fast Blue (FB), injected into the bladder wall prior to intravesical RTX or TTX administration. Immunocytochemical detection of LENK and VIP expression in the FB-labelled perikarya revealed that in the control group 25.15% of the FB-positive PCG neurons contained LENK, and 9.22% of them expressed VIP. Intravesical infusion of RTX resulted in an increase in the number of LENKIR neurons to 48.19% and VIP-IR perikarya to 11.25%. Optional treatment with TTX induced increase of LENK-IR neurons up to 81.67% and VIP-IR population to 16.46% of the FB-positive PCG cells. The present results show that both neurotoxins affect the chemical coding of PCG nervous cells supplying the porcine urinary bladder and that they stimulate both LENK and VIP expression. Furthermore, the results indicate a possible involvement of LENK and VIP neurons in the mechanisms of action of RTX and TTX in the therapy of overactive bladder disorder.
据报道，在旨在治疗膀胱功能障碍的多项临床试验中，树脂毒素（RTX）和河豚毒素（TTX）均有疗效。本实验的目的是研究膀胱内注射RTX和TTX对猪膀胱旁神经节（PCG）神经元化学编码的影响，这些神经元为膀胱提供神经支配。血管活性肠肽（VIP）和阿片样物质家族成员亮氨酸脑啡肽（LENK）在猪泌尿生殖道功能的调节作用方面均广为人知。在膀胱内注射RTX或TTX之前，通过向膀胱壁注射逆行示踪剂快蓝（FB）来识别支配膀胱的PCG神经元。对FB标记的胞体中LENK和VIP表达进行免疫细胞化学检测发现，在对照组中，25.15%的FB阳性PCG神经元含有LENK，其中9.22%表达VIP。膀胱内注入RTX导致LENK免疫反应性（LENK-IR）神经元数量增加至48.19%，VIP免疫反应性（VIP-IR）胞体增加至11.25%。TTX的选择性处理使LENK-IR神经元增加至FB阳性PCG细胞的81.67%，VIP-IR细胞群增加至16.46%。目前的结果表明，这两种神经毒素均影响供应猪膀胱的PCG神经细胞的化学编码，且它们均刺激LENK和VIP的表达。此外，结果表明LENK和VIP神经元可能参与了RTX和TTX治疗膀胱过度活动症的作用机制。
