Burliński Piotr Józef, Gonkowski Sławomir, Całka Jarosław
University of Warmia and Mazury in Olsztyn Department of Clinical Physiology, Faculty of Veterinary Medicine ul. Oczapowskiego 13/024 10-957 Olsztyn-Kortowo Poland.
Acta Vet Hung. 2011 Dec;59(4):455-63. doi: 10.1556/AVet.2011.033.
The aim of the present study was to establish the effect of intravesical administration of resiniferatoxin (RTX) and tetrodotoxin (TTX) on the chemical coding of paracervical ganglion (PCG) neurons supplying the urinary bladder in the pig. In order to identify the PCG neurons innervating the bladder, retrograde tracer Fast Blue was injected into the bladder wall prior to intravesical RTX or TTX administration. Consequent application of immunocytochemical methods revealed that in the control group 76.82% of Fast Blue positive PCG neurons contain nitric oxide synthetase (nNOS), and 66.92% contain acetylcholine transferase (ChAT). Intravesical infusion of RTX resulted in a reduction of the nNOS-IR neurons to 57.74% and ChAT-IR to 57.05%. Alternative administration of TTX induced an increase of nNOS-IR neurons up to 79.29% and a reduction of the ChAT-IR population down to 3.73% of the Fast Blue positive PCG cells. Our data show that both neurotoxins affect the chemical coding of PCG cells supplying the porcine urinary bladder, but the effects of their action are different. Moreover, these results shed light on the possible involvement of NO-ergic and cholinergic neurons in the mechanisms of therapeutic action exerted by RTX and TTX in curing the overactive bladder disorder.
本研究的目的是确定膀胱内给予树脂毒素（RTX）和河豚毒素（TTX）对猪膀胱副交感神经节（PCG）神经元化学编码的影响。为了识别支配膀胱的PCG神经元，在膀胱内给予RTX或TTX之前，将逆行示踪剂快蓝注入膀胱壁。随后应用免疫细胞化学方法显示，在对照组中，76.82%的快蓝阳性PCG神经元含有一氧化氮合酶（nNOS），66.92%含有乙酰胆碱转移酶（ChAT）。膀胱内注入RTX导致nNOS免疫反应性神经元减少至57.74%，ChAT免疫反应性神经元减少至57.05%。交替给予TTX导致nNOS免疫反应性神经元增加至79.29%，ChAT免疫反应性细胞群体减少至快蓝阳性PCG细胞的3.73%。我们的数据表明，两种神经毒素均影响支配猪膀胱的PCG细胞的化学编码，但其作用效果不同。此外，这些结果揭示了NO能和胆碱能神经元可能参与RTX和TTX治疗膀胱过度活动症的作用机制。
