Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan.
Med Sci Sports Exerc. 2013 Feb;45(2):238-44. doi: 10.1249/MSS.0b013e31826df4a1.
Neutrophil extracellular trap (NET), a newly revealed antimicrobial strategy, is usually evoked by reactive oxygen species (ROS) and nicotinamide adenine denucleotide phosphate (NADPH) oxidase activation. In addition, the acute severe exercise (ASE)-induced oxidative stress in neutrophils depends on the subject's physical fitness.
We investigated whether ASE exerted differential effects on NET formation in sedentary and physically active subjects.
Young males, 10 sedentary and 10 physically active, underwent an ASE (pedaling on a bicycle ergometer with increasing loads until exhaustion). Neutrophils were isolated from blood specimens drawn before and immediately after ASE for assaying NET formation along with redox-related parameters and mitochondrial membrane potential (ΔΨm).
In the sedentary group, (1) after ASE, NET formation increased spontaneously and in response to stimulation with phorbol 12-myristate 13-acetate; (2) ASE increased cytosolic ROS, decreased glutathione, and suppressed ΔΨm in neutrophils; (3) removing ROS or inhibiting NADPH oxidase prevented the ASE-facilitated NET formation; and (4) suppressing ΔΨm prevented the ASE-facilitated NET formation. On the contrary, these ASE effects on neutrophils did not happen in the active group.
ASE in sedentary but not active subjects facilitated NET formation via elevating the NADPH oxidase-generated ROS and suppressing the ΔΨm.
中性粒细胞细胞外陷阱（NET）是一种新发现的抗菌策略，通常由活性氧（ROS）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶激活引发。此外，中性粒细胞的急性剧烈运动（ASE）引起的氧化应激取决于受试者的身体健康状况。
我们研究了 ASE 是否对久坐和活跃的受试者的 NET 形成产生不同的影响。
10 名久坐的年轻男性和 10 名活跃的年轻男性进行 ASE（在自行车测力计上蹬踏，逐渐增加负荷直到力竭）。在 ASE 前后从血液样本中分离出中性粒细胞，以测定 NET 形成以及氧化还原相关参数和线粒体膜电位（ΔΨm）。
在久坐组中，（1）ASE 后，NET 形成自发增加，并对佛波醇 12-肉豆蔻酸 13-乙酸刺激产生反应；（2）ASE 增加了细胞质中的 ROS，减少了谷胱甘肽，并抑制了中性粒细胞中的 ΔΨm；（3）去除 ROS 或抑制 NADPH 氧化酶可防止 ASE 促进的 NET 形成；（4）抑制 ΔΨm 可防止 ASE 促进的 NET 形成。相反，在活跃组中，中性粒细胞没有发生这些 ASE 对中性粒细胞的影响。
ASE 在久坐而不是活跃的受试者中通过提高 NADPH 氧化酶产生的 ROS 和抑制 ΔΨm 促进 NET 形成。
