Department of Pharmacology, Key Laboratory of Antiinflammatory and Immunopharmacology of Education Ministry, Anhui Medical University, Hefei, China.
Phytother Res. 2013 Jun;27(6):869-76. doi: 10.1002/ptr.4793. Epub 2012 Aug 18.
Growing experimental and clinical data highlights the important roles of increased reactive oxygen species production in cardiovascular remodeling (CR). Oligomeric grape seed proanthocyanidins (GSPs) have been shown to be potent antioxidants and free radical scavengers. Mice were treated with DOCA-salt to induce CR and were given distilled water or oligomeric GSPs for 4 weeks. The heart weight (HW) index and kidney weight (KW) index were expressed as heart weight/body weight (HW/BW) and kidney weight/body weight (KW/BW); the histological changes were investigated by hematoxylin and eosin and Van Gieson staining.The endothelial-dependent vasodilation function induced by acetylcholine was investigated in isolated thoracic aorta ring. Colorimetric analysis was used to assay superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and nitric oxide (NO) content in serum and hydroxyproline content in cardiac tissue. Administration of GSPs markedly alleviated the elevation of HW/BW ratio, KW/BW ratio and cross-sectional area of cardiomyocytes, decreased collagen deposition in heart and attenuated histopathology injury, and improves endothelial-dependent aorta ring relaxation in vitro accompany by increasing of NO content in serum. Meanwhile, treatment with GSPs significantly ameliorated oxidative stress via increasing SOD activities and decreasing MDA formation. These findings suggest that administration of GSPs has the potential to attenuate DOCA-salt induced CR and KH and preserve NO activity and endothelial function, which mechanism may contribute to its antioxidant characteristic, at least in part.
越来越多的实验和临床数据强调了活性氧（ROS）产生增加在心血管重构（CR）中的重要作用。低聚原花青素（GSPs）已被证明具有强大的抗氧化和自由基清除能力。用 DOCA-盐处理小鼠以诱导 CR，并给予蒸馏水或低聚 GSPs 4 周。以心脏重量（HW）指数和肾脏重量（KW）指数表示心脏重量/体重（HW/BW）和肾脏重量/体重（KW/BW）；通过苏木精和伊红以及 Van Gieson 染色观察组织学变化。在分离的胸主动脉环中研究内皮依赖性乙酰胆碱诱导的血管舒张功能。比色分析法用于测定血清中超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量和一氧化氮（NO）含量以及心脏组织中羟脯氨酸含量。GSPs 的给药显著减轻了 HW/BW 比、KW/BW 比和心肌细胞横截面积的升高，减少了心脏中的胶原沉积并减轻了组织病理学损伤，并通过增加血清中 NO 含量改善了体外内皮依赖性主动脉环松弛。同时，GSPs 的治疗通过增加 SOD 活性和减少 MDA 形成显著改善了氧化应激。这些发现表明，GSPs 的给药具有减轻 DOCA-盐诱导的 CR 和 KH 的潜力，并维持 NO 活性和内皮功能，其机制至少部分归因于其抗氧化特性。
