Institute of Environmental Sciences, Bogazici University, Bebek, 34342 Istanbul, Turkey.
Bioprocess Biosyst Eng. 2013 May;36(5):541-6. doi: 10.1007/s00449-012-0809-y. Epub 2012 Aug 21.
The aim of this study was to investigate the effect of a common veterinary antibiotic in biogas plants. 20 mg/kg of oxytetracycline was intramuscularly injected into a cow and its concentration in manure, which was sampled daily during the following 20 days, was measured. A total of 20 % of the injected oxytetracycline was detected in manure. Collected manure samples on days 1, 2, 3, 5, 10, 15, and 20 were digested in triplicate serum bottles at 37 °C for 30 days. Control serum bottles produced 255 ± 13 mL biogas, whereas 50-60 % inhibitions were obtained for the serum bottles operated with samples collected for the 5 days after medication. Multivariate statistics used for the evaluation of FISH results showed that Methanomicrobiales were the main methanogenic group responsible for most of the biogas production. Numbers of active Bacteria and Methanomicrobiales were negatively correlated with the presence of oxytetracycline, whereas Methanosarcinales and Methanobacteriales were less affected.
本研究旨在探究一种常见兽医抗生素在沼气厂中的作用。将 20mg/kg 的土霉素给奶牛肌肉注射，随后在接下来的 20 天中每天采集粪便样本并测量其中的浓度。在粪便中检测到的土霉素总浓度为 20%。采集第 1、2、3、5、10、15 和 20 天的粪便样本，在 37°C 下于 3 个血清瓶中进行 30 天消化。对照血清瓶产生了 255 ± 13 mL 沼气，而在接种药物后 5 天采集的样本的血清瓶中，沼气产生量抑制了 50-60%。用于评估 FISH 结果的多变量统计表明，产甲烷菌目是主要的产甲烷菌群，负责大部分沼气的产生。活性细菌和产甲烷菌目数量与土霉素的存在呈负相关，而甲烷八叠球菌目和甲烷杆菌目受影响较小。
