Ozbayram E Gozde, Arikan Osman, Ince Bahar, Cetecioglu Zeynep, Aydin Sevcan, Ince Orhan
Environmental Engineering Department, Faculty of Civil Engineering, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey,
Environ Sci Pollut Res Int. 2015 Apr;22(8):6230-5. doi: 10.1007/s11356-014-3841-4. Epub 2014 Nov 20.
Pharmaceutical production industries are one of the main sources of antibiotics, and they release considerable amounts of antibiotics to ecosystem. Antibiotics usually present as mixtures in treatment plants and have negative effect on biological processes. In this study, batch acute tests were performed to assess the inhibitory impacts of selected antibiotic combinations of sulfamethoxazole and tetracycline (ST), erythromycin and sulfamethoxazole (ES), and erythromycin and tetracycline (ET) on acetoclastic methanogenic activity. Each antibiotic was equally applied, making the total concentrations in the mixtures 0 (control), 2, 20, 50, 100, 250, and 500 mg/L. Results showed decline characteristic on methane production with increasing antibiotic concentrations. EC50 values were calculated as 275 mg/L for ES, 219 mg/L for ST, and 130 mg/L for ET. Mixture inhibition of ST and ET combinations were accurately predicted using the concept of independent action, while ES combination resulted in almost the same inhibition with that of single antibiotic response. Inhibition on acetate utilization followed similar trend with methane production inhibition.
制药行业是抗生素的主要来源之一，它们向生态系统中释放了大量抗生素。抗生素在处理厂中通常以混合物形式存在，并且对生物过程有负面影响。在本研究中，进行了批次急性试验，以评估所选的磺胺甲恶唑和四环素（ST）、红霉素和磺胺甲恶唑（ES）以及红霉素和四环素（ET）抗生素组合对乙酸裂解产甲烷活性的抑制作用。每种抗生素均等量添加，使混合物中的总浓度分别为0（对照）、2、20、50、100、250和500mg/L。结果表明，随着抗生素浓度的增加，甲烷产量呈现下降特征。ES的EC50值计算为275mg/L，ST为219mg/L，ET为130mg/L。使用独立作用概念准确预测了ST和ET组合的混合抑制作用，而ES组合产生的抑制作用几乎与单一抗生素反应相同。对乙酸利用的抑制与甲烷生成抑制遵循相似趋势。
