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通过在皮肤缺损创面大鼠模型中移植颗粒状去细胞真皮基质实现成熟真皮的再生。

Regeneration of mature dermis by transplanted particulate acellular dermal matrix in a rat model of skin defect wound.

机构信息

Institute of Burn Research, Southwest Hospital, Third Military Medical University, 29 Gaotanyan Street, Chongqing, 400038, China.

出版信息

J Mater Sci Mater Med. 2012 Dec;23(12):2933-44. doi: 10.1007/s10856-012-4745-9. Epub 2012 Aug 19.

DOI:10.1007/s10856-012-4745-9
PMID:22903602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3506199/
Abstract

Native mammalian extracellular matrix (ECM) has been made in various forms including particles, sheet and mesh which are appropriate for site-specific applications. The ECM particles are usually created by homogenization method and have a wider size distribution. This needs to be improved to produce more uniform ECM particles. In present study, we had successfully developed a method for preparing particulate acellular dermal matrix (PADM) in different gauges. The resultant PADM was approaching a rectangular parallelepiped or cubic shape, with a better or narrower size distribution than other ECM particles in previous reports. It also retained ultrastructure and functional molecules of native ECM. In vivo performances were evaluated after implantation of PADM in an acute full-thickness skin defect wound in rats. Histological analysis showed that allogeneic PADM used as dermal regeneration template could facilitate maturation and improving collagen bundle structure of regenerated dermis at the endpoint of 20 weeks post-surgery. The PADM could be used for further investigation in analyzing the impacts of cellularly and/or molecularly modified PADM on soft tissue regeneration.

摘要

天然哺乳动物细胞外基质 (ECM) 已以各种形式制成，包括颗粒、薄片和网片，这些形式适用于特定部位的应用。ECM 颗粒通常通过匀浆法制成，具有较宽的粒径分布。这需要加以改进，以生产更均匀的 ECM 颗粒。在本研究中，我们成功开发了一种制备不同规格的细胞外脱细胞真皮基质 (PADM) 颗粒的方法。所得 PADM 接近长方体或正方体形状，与之前报道的其他 ECM 颗粒相比，粒径分布更好或更窄。它还保留了天然 ECM 的超微结构和功能分子。将 PADM 植入大鼠急性全层皮肤缺损创面后，对其体内性能进行了评估。组织学分析表明，用作真皮再生模板的同种异体 PADM 可促进术后 20 周时再生真皮的成熟和改善胶原束结构。可以进一步研究细胞和/或分子修饰的 PADM 对软组织再生的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/4be44d32cf14/10856_2012_4745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/9d9fc50accc9/10856_2012_4745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/3842f8f23704/10856_2012_4745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/0198b9be943d/10856_2012_4745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/e6dde161c390/10856_2012_4745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/4be44d32cf14/10856_2012_4745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/9d9fc50accc9/10856_2012_4745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/3842f8f23704/10856_2012_4745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/0198b9be943d/10856_2012_4745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/e6dde161c390/10856_2012_4745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ded/3506199/4be44d32cf14/10856_2012_4745_Fig5_HTML.jpg

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