Dang Song, Liu Qiaoling, Zhang Xuejie, He Kangmin, Wang Chunru, Fang Xiaohong
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
J Nanosci Nanotechnol. 2012 Jun;12(6):4478-84. doi: 10.1166/jnn.2012.6216.
This study provides a parallel cytotoxicity study of water-soluble single-walled carbon nanotubes and fullerene C70(C(COOH)2)4-8 on Nicotiana tabacum BY-2 cells. After BY-2 cells were treated with single-walled carbon nanotubes or C70(C(COOH)2)4-8, the cells adopted a shrunken morphology and exhibited lower proliferation rate. Fluorescence imaging of the treated cells revealed increased reactive oxygen species generation, abnormal mitochondrial distribution, decreased mitochondrial activity, and impaired actin cytoskeleton arrangement. However, no obvious cells death was induced by either single-walled carbon nanotubes or C70(C(COOH)2)4-8. Compared with single-walled carbon nanotubes, C70(C(COOH)2)4-8 exerted more serious adverse effects on BY-2 cells.
本研究对水溶性单壁碳纳米管和富勒烯C70(C(COOH)2)4-8对烟草BY-2细胞进行了平行细胞毒性研究。用单壁碳纳米管或C70(C(COOH)2)4-8处理BY-2细胞后，细胞形态收缩，增殖率降低。对处理后的细胞进行荧光成像显示，活性氧生成增加、线粒体分布异常、线粒体活性降低以及肌动蛋白细胞骨架排列受损。然而，单壁碳纳米管或C70(C(COOH)2)4-8均未诱导明显的细胞死亡。与单壁碳纳米管相比，C70(C(COOH)2)4-8对BY-2细胞的不利影响更严重。
