Department of Applied Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Forensic Sci Int. 2012 Oct 10;222(1-3):340-5. doi: 10.1016/j.forsciint.2012.07.014. Epub 2012 Aug 17.
A rapid quantitative colourimetric test was developed for the determination of 2,4,6-Trinitrotoluene (TNT) in soil. Quantification was achieved using an innovative application of photography where the relationship between the Red Green Blue (RGB) value and concentration of colorimetric product was exploited. The wide linear range (1-50 mg/L) and low detection limit (0.73±0.01 mg/L to 3.5±0.4 mg/L) achieved was comparable with spectrophotometric quantification methods. Quantification was validated both with known concentration standards and spiked soil extracts confirming the accuracy of the technique. The results demonstrate a powerful technique with the capacity for development as a rapid on site field test for the analysis of explosives.
一种快速定量比色法被开发用于测定土壤中的 2,4,6-三硝基甲苯(TNT)。通过创新的摄影应用实现了定量,利用比色产物的 RGB 值与浓度之间的关系。所达到的宽线性范围(1-50mg/L)和低检测限(0.73±0.01mg/L 至 3.5±0.4mg/L)与分光光度定量方法相当。通过已知浓度标准品和加标土壤提取物进行定量验证,证实了该技术的准确性。结果表明,该技术具有强大的潜力,可以开发为用于分析爆炸物的快速现场现场测试。