School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, Western Australia, 6009 Australia.
Muscle Nerve. 2012 Sep;46(3):413-20. doi: 10.1002/mus.23318.
The skeletal muscle weakness associated with many chronic diseases has been attributed to the catabolic effect of pro-inflammatory cytokines. We aimed to determine if local muscle inflammation has direct affects on contractile function and contributes to muscle weakness independent of muscle atrophy or mechanical injury.
Local muscle inflammation was induced by injecting an algal-derived polysaccharide, carrageenan (10 mg/kg), into the right tibialis anterior muscle in healthy ARC mice. The contralateral muscle was injected with sterile isotonic saline, and the muscles were removed after 24 h for measurement of contractile function and cytokine concentration.
Carrageenan significantly reduced maximum specific force, decreased the maximum rate of force development, altered the force-frequency relationship, and increased intramuscular levels of pro-inflammatory cytokines and chemokines.
These results indicate that carrageenan directly affects contractile function and causes skeletal muscle weakness. Local muscle inflammation may contribute to the weakness observed in inflammatory related disorders.
许多慢性疾病相关的骨骼肌无力归因于促炎细胞因子的分解代谢作用。我们旨在确定局部肌肉炎症是否对收缩功能有直接影响，并独立于肌肉萎缩或机械损伤导致肌肉无力。
将来源于海藻的多糖卡拉胶（10mg/kg）注入健康 ARC 小鼠的右胫骨前肌，以诱导局部肌肉炎症。将等渗盐水注入对侧肌肉，24 小时后取出肌肉，测量收缩功能和细胞因子浓度。
卡拉胶显著降低了最大比肌力，降低了最大力发展速率，改变了力频率关系，并增加了肌肉内促炎细胞因子和趋化因子的水平。
这些结果表明卡拉胶直接影响收缩功能并导致骨骼肌无力。局部肌肉炎症可能导致炎症相关疾病中观察到的无力。
